Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures, NEB

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Home > Technical Reference > Restriction Endonucleases > Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures



Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures

Listed below is the percentage activity exhibited at 37°C for enzymes that have an optimal incubation temperature higher (thermophiles) or lower (25°C) than 37°C.

Please also check these additional information about:
NEBuffer Activity | Double Digestion | Time-Saver Enzymes

Enzyme	Optimal Temperature	% Activity at 37°C
ApaI	25°C	100
ApeKI	75°C	10
ApoI	50°C	50
BaeI	25°C	20
BclI	50°C	50
BfuAI	50°C	50
BsaBI	60°C	20
BsaJI	60°C	20
BsaWI	60°C	20
BsiEI	60°C	30
BsiHKAI	65°C	5
BsiWI	55°C	50
BslI	55°C	30
BsmAI	55°C	50
BsmBI	55°C	20
BsmFI	65°C	50
BsmI	65°C	20
BspCNI	25°C	75
BspQI	50°C	10
BsrDI	65°C	30
BsrI	65°C	20
BssHII	50°C	75
BssKI	60°C	10
BstAPI	60°C	10
BstBI	65°C	10
BstEII	60°C	50
BstNI	60°C	30
BstUI	60°C	20
BstYI	60°C	30
BtgZI	60°C	75
BtsCI	50°C	50
BtsI	55°C	75
BtsIMutI	55°C	n/a
CviAII	25°C	20
CviQI	25°C	10
FatI	55°C	20
FauI	55°C	20
MwoI	60°C	10
Nb.BsmI	65°C	25
Nb.BsrDI	65°C	20
Nt.BspQI	50°C	80
Nt.BstNBI	55°C	10
PhoI	75°C	0
PI-PspI	65°C	5
PspGI	75°C	10
SfiI	50°C	10
SmaI	25°C	50
SmlI	55°C	10
SwaI	25°C	50
Taq^αI	65°C	10
TfiI	65°C	10
TliI	75°C	10
TseI	65°C	20
Tsp45I	65°C	10
Tsp509I	65°C	10
TspMI	75°C	20
TspRI	65°C	10
Tth111I	65°C	10

* ApaI has 100% activity at 37°C, however the half-life of this enzyme at 37°C is only 30 minutes.