New England Biolabs offers a wide variety of reagents to facilitate nucleic acid sample preparation for downstream applications including next-generation sequencing library construction and microarrays. Our comprehensive product selection includes restriction enzymes for DNA fragmentation, modification reagents to treat sample ends and enzymes to remove unwanted nuclear material. NEB carries a wide selection of DNA and RNA polymerases for amplification and kits for product detection. The following information highlights specific reagents currently available from New England Biolabs.

Fragmentation of DNA
NEB offers the largest selection of commercially available restriction enzyme specificities that can be used to generate libraries. This includes enzymes that cut within a specific recognition sequence, as well as those that cut outside of a recognition site. New enzymes are always being added through our restriction enzyme screening and cloning program. Cloning improves enzyme purity, resulting in the highest quality products with improved lot-to-lot consistency. In addition, our leading expertise in restriction enzyme research has enabled us to engineer High Fidelity Restriction Enzymes for several specificities. These enzymes exhibit vastly reduced star activity and ensure the specific cleavage required by many applications. We also offer DNase I and Micrococcal Nuclease for those preferring nonspecific cleavage.

End Treatments
NEB offers a wide variety of modification reagents for treating the ends of DNA or RNA following fragmentation (enzymatic or mechanical). Each of these enzymes is free of contaminating exonucleases and endonucleases.

Reagent	Fill in of 5´ Overhangs	Removal of 3´ Overhangs	Phosphate Removal	End Labeling	Notes
DNA Polymerase I	X	X
T4 DNA Polymerase	X	X
PreCR™ Repair Mix	X
Quick Blunting™ Kit	X	X	(3´)
Mung Bean Nuclease		X			for DNA or RNA; removes 5´ overhangs
Calf Intestinal Phosphatase			(3´, 5´)		for DNA or RNA
Antarctic Phosphatase			(3´, 5´)		for DNA or RNA
Terminal Transferase				X	labels the 3´ ends of DNA
Poly(A) Polymerase				X	labels RNA with cordycepin or ATP
T4 Polynucleotide Kinase			(3´)	X	labels the 5´ ends of DNA or RNA
T4 Polynucleotide Kinase (3´ phosphatase minus)				X	labels the 5´ ends of DNA or RNA
USER enzyme					creates a 3´ overhang

Removal of Unwanted Nuclear Material
Downstream applications often require the removal of unwanted nuclear material in order to maximize efficiency. Unwanted material can include single- or double-stranded DNA or RNA, DNA/RNA hybrids, oligonucleotides, and dNTPs. For removal reactions, NEB offers the following enzymes:

Reagent	Genomic DNA	dsDNA	ssDNA	dNTP	Other
DNase I	X	X	X		chromatin, DNA/RNA hybrids
Exonuclease I			X		oligonucleotides
Lambda Exonuclease	X	X			makes ssDNA
RecJf			X		dNMPs
Micrococcal Nuclease	X	X	X
Antarctic Phosphatase				X	also degrades pyrophosphate
McrBC	X				DNA containing methylcytosine
T7 Exonuclease			X		5´ mononucleotides from duplex DNA 5´ phosphorylated or dephosphorylated DNA DNA and RNA from DNA/RNA hybrids

= recombinant

Amplification Technologies
For the amplification of DNA or RNA, NEB offers a wide selection of polymerases and high quality reagents that can be used to maximize reaction yield. Our line of PCR reagents addresses a wide range of reaction conditions. Many polymerases are available as kits, master mixes and in a variety of optimized formats for added convenience. We also offer highly pure deoxynucleotides and a large selection of polymerases that can be used for specific amplification technologies.

* The Phusion product line includes Phusion™ Hot Start for enhanced specificity, and Phusion™ Flash for enhanced speed.
** The Taq product line includes several buffer formats and Crimson Taq™ in which the supplied buffer contains a crimson tracking dye for added convenience.

Detection Reagents
Often the final step in sample preparation is product detection. NEB provides many detection kits for various types of experiments, as well as reagents that can be used with other detection methods.

• DyNAmo™ qPCR Kits - sensitive quantification of DNA
  
• DyNAmo™ qRT-PCR Kits - sensitive quantification of RNA
  
• ProtoScript^® II RT-PCR Kit - detection of RNA
  
• NEBlot ^® Kit - produces labeled DNA probes of high specific activity suitable for use as hybridization probes for screening gene libraries
  
• Phototope^®-Star Detection Kit - chemiluminescent detection of nucleic acids
  
• NEBlot^® Phototope^® Kit - random primer biotin labeling to generate probes for sensitive nucleic acid detection
  
• Apyrase^®- catalyzes the removal of the gamma and beta phosphate from ATP
  
• ATP Sulfurylase^®- catalyzes the activation of sulfate by transferring sulfate to the adenine monophosphate moiety of ATP to form APS and PPi

All reagents for sample preparation can be produced in large scale with stringent specifications that ensure success for your high throughput technologies.

For more information regarding customization and OEM opportunities, please contact oem@neb.com.