An optimized blend of Taq and Deep Vent_R^® DNA polymerases, OneTaq® and OneTaq® Hot Start DNA Polymerases offer robust amplification across a wide range of templates. The 3´–5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robustness of Taq, and the hot start formulation combines convenience with decreased interference from primerdimers and secondary products. Additionally, OneTaq Reaction Buffers and High GC Enhancer have been formulated for robust yields with minimal optimization, regardless of a template's GC content. Both polymerases are available in master mix and Quick-Load^® master mix formats.

In contrast to chemically modified or antibody-based hot start polymerases, NEB's OneTaq Hot Start utilizes aptamer technology. This unique modified oligonucelotide binds to the polymerase through non-covalent interactions, blocking polymerase activity at temperatures below 45°C. The polymerase is activated during normal cycling conditions, allowing reactions to be set up at room temperature.


Advantages:

• Exceptional performance in endpoint PCR across a wide range of template
• Robust yields with minimal optimization
• Convenient product formats (stand-alone enzyme, master mixes, and Quick-Load^® formats)
• Hot start version allows room temperature reaction setup and does not require a separate activation step
• Compatible with standard Taq protocols

• High sensitivity PCR
• High throughput PCR
• Routine PCR
• GC-rich PCR
• AT-rich PCR
• Primer extension
• Colony PCR
• Long PCR (up to ~6 kb genomic)

Achieve robust amplification for routine, AT- and GC-rich
templates with OneTaq

Amplification of a selection of sequences with varying AT and GC
content from human and C. elegans genomic DNA using OneTaq
DNA Polymerase. GC content is indicated above gel. Marker M is
the 1 kb DNA Ladder.


Comparison of OneTaq DNA Polymerase to other commercially available
polymerases (non-hot start).

Amplification of a selection of high GC human genomic DNA templates
demonstrates OneTaq performance. PCR experiments included 30 amplification
cycles. Purity (A) and Yield (B) were calculated via microfluidic analysis from
triplicate reactions. Competitor polymerases were cycled according to
manufacturer's recommendations.

OneTaq Hot Start DNA Polymerase

Amplification of a selection of sequences with varying GC content from human
and C. elegans genomic DNA using OneTaq Hot Start DNA Polymerase. The
presence or absence of an extended room temperature incubation does not
affect performance. GC content is indicated above gel. Marker M is the 1 kb
DNA Ladder.

OneTaq 2X Master Mix with Standard Buffer Amplification of a selection of sequences with varying GC content from human and C. elegans genomic DNA using OneTaq 2X Master Mix with Standard Buffer. Amplicon sizes are indicated next to gel, and GC content is indicated above gel. Marker M is the 1 kb DNA Ladder.

OneTaq 2X Master Mix with GC Buffer Amplification of a selection of sequences with varying GC content from human genomic DNA using OneTaq 2X Master Mix with GC Buffer. GC content is indicated above gel. Marker M is the 1 kb DNA Ladder.

Comparison of OneTaq Hot Start DNA Polymerases to other commercially available hot start polymerases.

Reactions containing high GC human genomic DNA templates were set up at room temperature. PCR experiments included 30 cycles.
Purity (A) and Yield (B) were calculated via microfluidic analysis from triplicate reactions. OneTaq polymerases were used with GC
Buffer. Some OneTaq reactions also contained High GC Enhancer (striped bars). Competitor polymerases were cycled according to
manufacturer's recommendations and included GC enhancers when supplied (striped bars).