 |
|
 |
|
|
|
| |
Vent |
Vent
(exo-) |
Deep
Vent |
Deep
Vent
(exo-) |
Taq |
9°Nm |
Phusion |
DyNA-
zyme
EXT |
DyNA-
zymeII
Hot Start |
| Exonuclease
Activities |
3'—>5'
proofreading
exonuclease |
yes |
no |
yes |
no |
no |
1-5% |
yes |
yes |
no |
5'—>3'
exonuclease
activity |
no |
no |
no |
no |
yes |
no |
no |
yes |
yes |
| Thermal
Stability |
| Half
life at 95°C |
6.7
hours |
6.7
hours |
23
hours |
23
hours |
0.9
hours |
6.7
hours |
>6hours
at 96°C |
3.5 hours
at 96°C |
0.9 hours |
| Half
life at 100°C |
1.8
hours |
1.8
hours |
8
hours |
8
hours |
<0.1
hours |
1.8
hours |
2 hours
at 98°C |
|
<0.1 hours |
| Primer
Extension Characteristics |
Units/100
µl
Primer Extension Rxn |
1-2
units |
2-4
units |
1-2
units |
2-4
units |
2-5
units |
1-2
units |
1-2 units |
1-4 units |
1-4 units |
Error
Rate
(x10-6 base insertions) |
57 |
190 |
~20 |
~190 |
285 |
in
prograss |
.44 |
|
|
| Resulting
DNA Ends |
blunt ends |
3´ A |
blunt ends |
3´ A |
3´
A |
3´ A
blunt |
blunt |
3´ A
blunt |
3´ A |
| Strand
Displacement |
yes;
temp.
dependent |
yes;
temp.
dependent |
yes;
temp.
dependent |
yes;
temp.
dependent |
no |
yes
|
no |
yes;
temp.
dependent |
no |
Longest
Primer
Extension to Date |
13.2
kb |
15.0
kb |
14.0
kb |
15.0
kb |
8.1
kb |
14.0
kb |
37 kb |
40 kb |
3 kb |
|
|
 |
|
