 |
|
 |
|
|
Bacterial Cells:
E. coli or Salmonella typhimurium
| Cell
Data |
per
cell |
per
liter at 109
cells per ml |
| Wet
Weight |
9.5
X 10-13
g |
0.95
g |
| Dry
Weight |
2.8
X 10-13
g |
0.28
g |
| Total
Protein |
1.55
X 10-13
g |
0.15
g |
| Volume |
1.15
µm3
= 1 femtoliter |
|
| Protein
Conc. in the cell: 135 mg/ml |
Theoretical
maximum yield for a 1 liter culture (109
cells /ml) if protein of interest is:
0.1%
of total protein: 150
µg/liter
2.0% of total protein: 3
mg/liter
50.0% of total protein: 75
mg/liter |
|
|
