SNAP-Cell       SNAP-Surface        CLIP-Cell        CLIP-Surface        ACP- & MCP- Surface

To enable researchers to quickly and easily begin using our protein labeling system, our Starter Kits include all the components necessary to covalently attach either a red or a green fluorophore to SNAP-tag®, CLIP-tag™ or ACP-tag fusion in living cells, fixed cells or in vitro. Each Starter Kit contains a plasmid encoding the tag of interest and two cell permeable or non-cell permeable fluorescent labels. A positive control plasmid encoding a tagged protein with a well-charaterized subcellular localization is also included (e.g., membrane, nucleus, etc.). Where available, a negative control blocking agent is included that interacts with the tag of interest, but is non-fluorescent.

			SNAP-Cell™: Cell-permeable Labels spread across the visible spectrum, ranging from blue to red Non-fluorescent cell-permeable blocking agent available
			SNAP-Surface™: Non-cell permeable Label SNAP-tag fusion proteins on the surface of living cells, in fixed cells or in vitro Labels spread across the visible and near-infrared spectrum including photostable AlexaFluor® dyes Non-fluorescent blocking agent available Use SNAP-Vista Blue or Green to visualize proteins directly in SDS-PAGE
			CLIP-Cell™: Cell-permeable Label CLIP-tag fusion proteins inside living cells, on cell surfaces or in vitro Simultaneous labeling of 2 expressed proteins when used with SNAP-tag Labels, ranging from blue to red Non-fluorescent cell-permeable blocking agent available
			CLIP-Surface™: Non-cell permeable Label CLIP-tag fusion proteins on the surface of living cells, in fixed cells or in vitro Simultaneous labeling of 2 expressed proteins when used with SNAP-tag Labels include fluorophores at commonly used areas of the visible spectrum (488, 547 and 647nm)
			ACP- & MCP- Surface: Require a specific synthase to label their protein target using ACP Synthase or SFP Synthase