Overview            Features             Applications   

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  Press play to view the video "Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging"

SNAP- and CLIP-tag protein labeling systems enable the specific, covalent attachment of virtually any molecule to a protein of interest. There are two steps to using this system: cloning and expression of the protein of interest as a SNAP-tag® fusion, and labeling of the fusion with the SNAP-tag substrate of choice. The SNAP-tag is a small protein based on human O⁶-alkylguanine-DNA-alkyltransferase (hAGT), a DNA repair protein. SNAP-tag labels are dyes conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag. CLIP-tag™ is a modified version of SNAP-tag, engineered to react with benzylcytosine rather than benzylguanine derivatives. When used in conjunction with SNAP-tag, CLIP-tag enables the orthogonal

Live COS-7 cells transfected with pSNAP-tubulin. Cells were labeled with SNAP-Cell TMR- Star (green pseudocolor) for 30 minutes and counterstained with Hoescht 33342(blue) for nuclei.	Features: Clone and express once, then use with a variety of fluorescent labels Easy-to-use protocols enable rapid labeling with a single reagent Optimized for a range of imaging instrumentation Non-toxic to living cells Wide selection of labels Highly specific covalent labeling Simultaneous dual labeling Easy-to-use starter kits available
Live NIH3T3 cells transfected with pSNAP-Actin. Cells were labeled with SNAP-Cell TMR- Star (green pseudocolor) for 30 minutes and counterstained with Hoechst 33342 (blue) for nuclei.	Applications: Simultaneous dual protein labeling inside live cells Protein localization and translocation Pulse-chase experiments Receptor internalization studies Selective cell surface labeling Protein pull down assays Protein detection in SDS-PAGE Flow cytometry High throughput binding assays in microtiter plates Biosensor interaction experiments FRET-based binding assays