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Fran Perler
Senior Scientist
Chemical Biology
perler@neb.com
978-380-7326
Curriculum Vitae
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| My group works on various aspects of intein-mediated protein splicing. We study the mechanism of protein splicing, intein motifs, intein evolution and intein applications. I also curate InBase, the on-line intein database. Previously, I cloned Vent DNA polymerase and my group has cloned and characterized several other archaeal DNA polymerases. |
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| Selected Publications: |
- Perler, F. B., Comb, D. G., Jack, W. E., Moran, L. S., Qiang, B., Kucera, R. B., Benner, J., Slatko, B. E., Nwankwo, D. O., Hempstead, S. K., and et al. (1992). Intervening sequences in an Archaea DNA polymerase gene. Proc Natl Acad Sci U S A 89: 5577-81.
- Xu, M. Q., Southworth, M. W., Mersha, F. B., Hornstra, L. J., and Perler, F. B. (1993). In vitro protein splicing of purified precursor and the identification of a branched intermediate. Cell, 75: 1371-7.
- Southworth, M.W., Adam, E., Panne, D., Byer, R., Kautz. R., and Perler, F.B. (1998) Control of Protein Splicing by Intein Fragment Reassembly. Embo J. 17: 918-26.
- Southworth, M.W., Benner, J. and Perler, F.B. (2000). An Alternative Protein Splicing Mechanism for Inteins Lacking an N-terminal Nucleophile. EMBO J. 19: 5019-5026.
- Perler, F.B. (2002). InBase, The Intein Database. Nuc. Acids Res. 30: 383-384.
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