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N0363S |
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25 gel lanes
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500 μg/ml |
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Description:
The dsRNA Ladder is a set of 7 annealed double-stranded RNA molecules produced by in vitro transcription of 14 linear DNA templates. The ladder sizes are: 500, 300, 150, 80, 50, 30 and 21 base pairs. All have 2-base, 3´ extensions. The 80 base pair fragment is at double intensity to serve as a reference point.
This ladder is suitable for use as a size standard in dsRNA and RNAi analysis on both non-denaturing polyacrylamide and agarose gels.
Left: 1 µg of dsRNA Ladder was visualized by ethidium bromide staining on a 6% Polyacrylamide gel 10 V/cm.
Right: 1.5 µg of dsRNA Ladder was visualized by ethidium bromide staining on a 2% TBE agarose gel 10 V/cm.
Concentration:
500 μg/ml
Storage Conditions
Storage Conditions:
10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C
Storage Temperature:
-20°C
Notes
General notes:- Double-stranded RNA is sensitive to degradation by ribonucleases, even though it is more resistant than single-stranded RNA.
To avoid ribonuclease contamination: wear gloves, use RNase-free water for gels and buffers, wash equipment with detergent and rinse throughly with RNase-free water.
Excessively long run times or high voltage can cause degradation of the bands on the gel. We recommend loading 1.0-1.5 µg of dsRNA Ladder, a running voltage of 4-10 V/cm , adding ethidium bromide to gels and running buffer at a final concentration of 0.5 µg/ml to effectively stain the bands during electrophoresis.
Usage notes:- This marker was not designed for precise quantification of dsRNA mass.
References
- Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, (2nd ed.), (p. 7.43?7.45), Cold Spring Harbor Laboratory Press.
- Liu, Y-C. and Chou, Y-C. (1990) Biotechniques, 9, 558.
- Dong Ma, New England BioLabs, Inc., unpublished observations.
Companion Products
HiScribe RNAi Transcription Kit
ProtoScript® First Strand cDNA Synthesis Kit
ShortCut® RNAi Kit
siRNA Marker
ssRNA Ladder
T7 RNA Polymerase
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