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| | | | E. coli DNA Ligase Reaction Buffer | | | |
Description:
New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme.
Reagents Supplied:
10X E. coli DNA Ligase Reaction Buffer
1X E. coli DNA Ligase Reaction Buffer:
30 mM Tris-HCl
4 mM MgCl2
1 mM Dithiothreitol
26 µM NAD
50 µg/ml BSA
pH 8.0 @ 25°C
Storage Conditions
Storage Temperature:
-20°C
FAQs
- What is supplied with the E.coli DNA Ligase Reaction Buffer pack?
- What is the composition of E.coli DNA Ligase Reaction Buffer?
- How should the NEBuffer be used?
- The buffer arrived thawed; is it and the enzyme still active?
Quality Control for Current Lot
Quality control values for a specific lot can be found on the datacard which accompanies each product.
16-Hour Incubation::
A 50 µl reaction containing this reaction buffer at a 1X concentration and 1 µg of HaeIII digested ΦX174 RF I DNA incubated for 16 hours resulted in no detectable non-specific nuclease degradation.
Endonuclease Activity::
Incubation of this reaction buffer at a 1X concentration with 1 µg ΦX174 RF I DNA for 4 hours at 37°C in 50 µl reactions resulted in less than 5% conversion to RF II.
Companion Products
E. coli DNA Ligase
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