Q2: How does HhaI deffer from its isoschizomer, HinP1I?
A2: HhaI produces a 3´ extension whereas HinP1I produces a 5´ extension.
Q3: Does HhaI cleave single-stranded DNA?
A3: Yes, it cleaves single-stranded DNA at about 50% the rate of double-stranded DNA. It is unclear if the enzyme cuts single stranded DNA or at double stranded regions in single stranded DNA.
