A1: The molecular weight of the NEB Endonuclease V is 80 kDa because it is produced as an MBP fusion (MBP MW = 55kDa)
Q2: What substrate is used to test Endonuclease V activity?
A2: The susbstrates we use to test enzyme activity are listed under the unit definition of each enzyme. In the case of Endonuclease V a 34-mer oligonucleotide duplex containing a single deoxyinosine site is used.
Q3: What is the activity of Endonuclease V in the different NEBuffers?
A3: The activity of Endonuclease V in the NEBuffers is as follows: NEBuffer 1: 100% activity; NEBuffer 2: 100% activity; NEBuffer 3: 75% activity; NEBuffer 4: 100% activity.
Q4: How well does Endonuclease V cleave at U:A sites?
A4: Endonuclease V does not work well at U:A sites. However, it will cleave the phosphodiester bond 3' to a deoxyuridine mismatch if it is a U:G mismatch. For additional information please refer to the "DNA Repair Glycosylases on Various Damages Bases" page in our website.
Q5: Can Endonuclease V cleave the loop in a stem-loop DNA structure?
A5: Yes, Endonuclease V has been reported to be able to cleave the loop in a stem-loop DNA structure.
Q6: Can a nick generated by Endonuclease V activity be repaired with DNA ligase?
A6: Yes, a nick generated by Endonuclease V can be ligated by DNA ligase.
Q7: Can Endonuclease V recognize and nick mismatches?
A7: Yes. Endonuclease V will recognize mismatches. However, the efficiency of the nicking in these sites is very low.
