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N0316S |
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0.04 ml
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1 x1013 pfu/ml |
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Description:
M13KE Phage is a suspension of infectious virions derived from the Ph.D. cloning vector, M13KE (1). The vector phage may be a useful control for phage ELISA or for titering of phage stocks. For cloning, the isolated replicative form of M13KE is available as part of the Ph.D. Peptide Display Cloning System (NEB #E8101S).
Source:
M13KE phage was isolated from infected E. coli ER2738 by a standard procedure (2).
Reaction & Storage Conditions
Concentration:
1 x1013 pfu/ml
Storage Conditions:
50% Glycerol
1 X TBS
Storage Temperature:
-20°C
Protocols
Protocols for M13KE Phage
Quality Control for Current Lot
Quality control values for a specific lot can be found on the datacard which accompanies each product.
Absolute titer:
Infection of a mid-log culture of E. coli ER2738 followed by plating yielded 1.0 x 1013 pfu/ml.
Sequence verification:
Sequencing of M13KE was carried out with -96 glll Sequencing Primer (20-mer) (NEB #S1259S). Single digests of M13KE vector by KpnI and EagI were carried out to confirm presence of cloning sites.
Wild-type M13 contamination:
A plate with >103 blue plaques showed no white plaques.
References
- Noren, K. A. and Noren, C. J. (2001) Methods, 23, 169-178.
- Sambrook, J. and Russell, D. W. (2001) Molecular Cloning: A Laboratory Manual, (3rd ed.), (pp3.17-3.32), Cold Spring Harbor: Cold Spring Harbor Laboratory .
Companion Products
Ph.D.™ Peptide Display Cloning System
Ph.D.™-12 Phage Display Peptide Library Kit
Ph.D.™-7 Phage Display Peptide Library Kit
Ph.D.™-C7C Phage Display Peptide Library Kit
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