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CP Reaction Buffer Protocol 1: Western Blotting |  | | CP Reaction Buffer Protocol 1: Western Blotting |
Protocol
- Dissolve your peptide in water to a concentration of 1 mM.
- Thaw the carrier protein at room temperature.
- Mix the following (in order):
- Incubate at room temperature for 15-60 minutes or at 4°C overnight.
- Add 12.5 µl of 3X SDS Sample Buffer.
- Heat at 95°C for 5 minutes.
- Load 5-10 µl/lane (~ 25-50 ng) for Western blotting.
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