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Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE

Home > Products > Carrier Protein 27 (Western) protocol 2: Analysis of Ligation by Coomassie Blue Stained SDS-PAGE Carrier Protein 27 (Western) protocol 2: Analysis of Ligation by Coomassie Blue Stained SDS-PAGE Protocol Dissolve your peptide in water to a concentration of 1 mM Thaw the carrier protein at room temperature. Mix the following (in order): Incubate at room temperature for 15-60 minutes or at 4°C overnight. Add 12.5 µl of 3X SDS Sample Buffer. Heat at 95°C for 5 minutes. Load 15 µl/lane (~0.8 µg carrier protein) for Coomassie Blue staining. Dilute 10-fold and load 10 µl (~53 ng) for Western blot analysis.