To access your account, log in or register.	shopping cart | log in

Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE

Home > Products > Carrier Protein 27 (Western) Protocol 1: For Western Blotting Carrier Protein 27 (Western) Protocol 1: For Western Blotting Protocol Dissolve your peptide in water to a concentration of 1 mM. Thaw the carrier protein at room temperature. Mix the following (in order): Incubate at room temperature for 15-60 minutes or at 4°C overnight. Add 12.5 µl of 3X SDS-PAGE Sample Buffer. Heat at 95°C for 5 minutes. Load 5-10 µl/lane (~25-50 ng) for Western blotting.