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Protocols for Protein G Magnetic Beads FAQs for Protein Expression and Purification Technical Reference for Protein Expression and Purification Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder PCR Selection Tool Isoschizomers DNA Sequences and Maps REBASE 6-Tube Magnetic Separation Rack Protein A Magnetic Beads Streptavidin Magnetic Beads

Home > Products > Protein Expression and Purification > Magnetic Matrices and Racks > Protein G Magnetic Beads Protein G Magnetic Beads Catalog # Size Concentration Price Qty   S1430S 1 ml   $161.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Small-scale purification or immunoprecipitation of IgG species No centrifugation required Regenerate matrix without binding capacity loss Description: Protein G Magnetic Beads are an affinity matrix for the small-scale isolation and purification of immunoglobulins. A truncated form of recombinant Protein G is covalently coupled to a nonporous paramagnetic particle. Protein G exhibits high affinity for subclasses of IgG from many species including human, rabbit, mouse, rat and sheep (1). In addition, Protein G can be used for immunoprecipitations with mouse monoclonal antibodies (1). The protein is coupled through a linkage that is stable and leak resistant over a wide pH range. This permits the immunomagnetic purification of IgGs from ascites, serum or cell culture supernatants; the matrix can then be regenerated without loss of binding capacity. Protein G Magnetic Beads can be used to immunoprecipitate target proteins from crude cell lysates using selected primary antibody. In addition, specific antibodies can be chemically cross-linked to the Protein G coated surface to create a reusable immunoprecipitation bead, avoiding the co-elution of antibody with target antigen (2,3). Advantages: No centrifugation required; matrix can be regenerated without loss of binding capacity. Minimal sample loss during pipetting because magnetic beads concentrate at the side of the tube instead of the bottom. Protein A and Protein G magnetic beads allow for the small-scale purification or immunoprecipitation of IgG species. Supplied As: Liquid Properties Ligand Binding Capacity Human IgG 400 μg/ml Storage Conditions Storage Conditions: 155 mM NaCl 0.02% sodium azide 0.1% BSA 0.05% Tween-20 2.97 mM Na2HPO4 1.06 mM KH2PO4 pH 7.2 @ 25°C Storage Temperature: 4°C Notes General notes: Support Matrix: 2 µm nonporous superparamagnetic microparticles. 1 ml of Protein G Magnetic Beads will bind > 400 µg of Human IgG. Protocols Protocols for Protein G Magnetic Beads References Harlow, E. and Lane, D. (1988) Bacterial Cell Wall Proteins that Bind Antibodies. Antibodies: A Laboratory Manual, 615-619. Schneider, C., Newman, R.A. et al. (1982) J. Biol. Chem., 257, 10766. Sisson, T.H. and Castor, C.W. (1990) J. Immunol. Methods, 127, 215. Companion Products 6-Tube Magnetic Separation Rack Protein A Magnetic Beads Streptavidin Magnetic Beads New England Biolabs, Inc. is an ISO 9001 and ISO 14001 Certified Company. NEB certifies that it is a small business in accordance with the US Small Business Administration and 13 CFR 121.201