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Protein G Magnetic Beads |
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Prices are in US dollars and valid only for US orders.
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- Small-scale purification or immunoprecipitation of IgG species
- No centrifugation required
- Regenerate matrix without binding capacity loss
Description:
Protein G Magnetic Beads are an affinity matrix for the small-scale isolation and purification of immunoglobulins. A truncated form of recombinant Protein G is covalently coupled to a nonporous paramagnetic particle. Protein G exhibits high affinity for subclasses of IgG from many species including human, rabbit, mouse, rat and sheep (1). In addition, Protein G can be used for immunoprecipitations with mouse monoclonal antibodies (1). The protein is coupled through a linkage that is stable and leak resistant over a wide pH range. This permits the immunomagnetic purification of IgGs from ascites, serum or cell culture supernatants; the matrix can then be regenerated without loss of binding capacity. Protein G Magnetic Beads can be used to immunoprecipitate target proteins from crude cell lysates using selected primary antibody. In addition, specific antibodies can be chemically cross-linked to the Protein G coated surface to create a reusable immunoprecipitation bead, avoiding the co-elution of antibody with target antigen (2,3).
Advantages:- No centrifugation required; matrix can be regenerated without loss of binding capacity.
- Minimal sample loss during pipetting because magnetic beads concentrate at the side of the tube instead of the bottom.
- Protein A and Protein G magnetic beads allow for the small-scale purification or immunoprecipitation of IgG species.
Supplied As: Liquid
Properties
Storage Conditions
Storage Conditions:
155 mM NaCl
0.02% sodium azide
0.1% BSA
0.05% Tween-20
2.97 mM Na2HPO4
1.06 mM KH2PO4
pH 7.2 @ 25°C
Storage Temperature:
4°C
Notes
General notes:- Support Matrix: 1 µm nonporous superparamagnetic microparticle.
- 1 ml of Protein G Magnetic Beads will bind > 400 µg of Human IgG.
References
- Harlow, E. and Lane, D. (1988) Bacterial Cell Wall Proteins that Bind Antibodies. Antibodies: A Laboratory Manual, 615-619.
- Schneider, C., Newman, R.A. et al. (1982) J. Biol. Chem., 257, 10766.
- Sisson, T.H. and Castor, C.W. (1990) J. Immunol. Methods, 127, 215.
Companion Products
6-Tube Magnetic Separation Rack
Protein A Magnetic Beads
Streptavidin Magnetic Beads
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