 |
|
 |
|
|
| Home >
Products >
Gene Expression and Protein Purification >
Magnetic Matrices and Racks >
Oligo d(T)25 Magnetic Beads |
 | | | | Oligo d(T)25 Magnetic Beads | | | |
 |
|
Prices are in US dollars and valid only for US orders.
|
Description:
Oligo d(T)25 Magnetic Beads are a 1 μm polymer based affinity matrix for the small-scale isolation of mRNA from crude cell lysates and tissue. The isolation occurs through the hybridization of covalently coupled oligo d(T)25 to the poly(A) region present in most eukaryotic mRNA. Applications include direct mRNA isolation following lysis and second-round purification of previously isolated total RNA. The magnetic separation technology is scalable and permits elution of intact mRNA in small volumes eliminating the need for precipitation of the isolated mRNA. Beads can be reused up to three times and the researcher has the option of eluting the isolated mRNA or using the bound d(T)25 as a primer in a first-strand cDNA reaction.
Support Matrix: 1 μm nonporous superparamagnetic microparticles
Recommended Buffers:
Lysis/Binding Buffer:
20 mM Tris-HCl (pH 7.5)
500 mM LiCl
0.5% LiDS
1 mM EDTA
5 mM DTT
Wash Buffer I:
20 mM Tris-HCl (pH 7.5)
500 mM LiCl
0.1% LiDS
1 mM EDTA
5 mM DTT
Wash Buffer II:
20 mM Tris-HCl (pH 7.5)
500 mM LiCl
1 mM EDTA
Low Salt Buffer:
20 mM Tris-HCl (pH 7.5)
200 mM LiCl
1 mM EDTA
Elution Buffer:
20 mM Tris-HCl (pH 7.5)
1 mM EDTA
Properties
Storage Conditions
Storage Conditions:
1 X PBS
0.1% Tween-20
0.02% NaN3
pH 7.4 @ 25°C
Storage Temperature:
4°C
Companion Products
12-Tube Magnetic Separation Rack
6-Tube Magnetic Separation Rack
DyNAmo™ SYBR® Green 2-Step qRT-PCR Kit
ProtoScript® First Strand cDNA Synthesis Kit
ProtoScript® II RT-PCR Kit
|
|
|
 |
|
|
