 | | | | 3´-0-Me-7mG(ppp)G RNA Cap Structure Analog | | | |
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Prices are in US dollars and valid only for US orders.
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Description:
Blocking of the 3´ -hydroxyl of 7mG with 3´-0-Me assures that the capped transcripts are homogeneous. The 3´ - hydroxyl of the non-methylated G is the only 3´ - hydroxyl available for initiation (1).
The 5´ terminal m7G cap present on most eukaryotic mRNAs promotes translation in vitro at the initiation level (2,3,4). For most RNAs, the cap structure increases causes a loss of stability, especially against exonuclease degradation (5), and a decrease in the formation of the initiation complex of mRNAs for protein synthesis (5,6). Certain prokaryotic mRNAs containing a 5´ terminal cap structure are translated as efficiently as or more efficiently than eukaryotic mRNAs in a eukaryotic cell-free protein synthesizing system (6). Also a cap requirement has been observed for splicing eukaryotic substrate RNAs (7).
A method using E. coli RNA Polymerase primed with m7G(5´)ppp(5´)G or m7G(5´)ppp(5´)A for an efficient in vitro synthesis of capped RNAs has been developed by Contreas (8). Larger amounts of capped RNAs are produced by transcription systems using SP6 RNA Polymerase primed with m7G(5´)ppp(5´)G (7).
Sequence: 3'-O-Me-7mG(5')ppp(5')G
Properties & Usage
Supplied As: Sodium Salt
Suggested Working Concentration: 10 mM
Recommended Resuspension Volume: 131 μl
Molecular Weight:
Theoretical: 817 daltons
Storage Conditions
Storage Temperature:
-20°C
Notes
General notes:- Addition of 131 µl water gives approximately a 10 mM solution.
References
- Peng, Z.-H. et al. (2002) ORG. LETT., 4(2).
- Shatkin, A. J. (1978) Cell, 9, 645-653.
- Fillipowicz, W. (1978) FEBS Lett, 96, 1-11.
- Banerjee, A.K. (1980) Microbiol. Rev., 44, 175-205.
- Miura, K. (1981) Adv. Biophys., 14, 205-238.
- Shatkin, A. J. et al. (1977) Nucleic Acids. Res., 4, 3065-3081.
- Konarska, M. M. et al. (1984) Cell, 38, 731-736.
- Contreas, R. et al. (1982) Nucleic Acids. Res., 10, 6353-6363.
- Paterson, B. M. and Rosenberg, M. (1979) Nature, 279, 696-701.
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