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Home > Products > RNAi and RNA Enzymes > RNA Isolation and Analysis > RNA Cap Structure Analogs > 3´-0-Me-m7G(5')ppp(5')G RNA Cap Structure Analog 3´-0-Me-m7G(5')ppp(5')G RNA Cap Structure Analog Catalog # Size Concentration Price Qty   S1411L 125 A260 units   $680.00 S1411S 25 A260 units   $170.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: Blocking of the 3´ -hydroxyl of m7G with 3´-0-Me assures that the capped transcripts are homogeneous. The 3´ - hydroxyl of the non-methylated G is the only 3´ - hydroxyl available for initiation (1). The 5´ terminal m7G cap present on most eukaryotic mRNAs promotes translation in vitro at the initiation level (2,3,4). For most RNAs, the cap structure increases causes a loss of stability, especially against exonuclease degradation (5), and a decrease in the formation of the initiation complex of mRNAs for protein synthesis (5,6). Certain prokaryotic mRNAs containing a 5´ terminal cap structure are translated as efficiently as or more efficiently than eukaryotic mRNAs in a eukaryotic cell-free protein synthesizing system (6). Also a cap requirement has been observed for splicing eukaryotic substrate RNAs (7). A method using E. coli RNA Polymerase primed with m7G(5´)ppp(5´)G or m7G(5´)ppp(5´)A for an efficient in vitro synthesis of capped RNAs has been developed by Contreas (8). Larger amounts of capped RNAs are produced by transcription systems using SP6 RNA Polymerase primed with m7G(5´)ppp(5´)G (7). Sequence: 3'-O-Me-7mG(5')ppp(5')G Properties & Usage Supplied As: Sodium Salt Suggested Working Concentration: 10 mM Recommended Resuspension Volume: 131 μl Molecular Weight: Theoretical: 817 daltons Storage Conditions Storage Temperature: -20°C Notes General notes: Addition of 131 µl water gives approximately a 10 mM solution. References Peng, Z.-H. et al. (2002) ORG. LETT., 4(2). Shatkin, A. J. (1978) Cell, 9, 645-653. Fillipowicz, W. (1978) FEBS Lett, 96, 1-11. Banerjee, A.K. (1980) Microbiol. Rev., 44, 175-205. Miura, K. (1981) Adv. Biophys., 14, 205-238. Shatkin, A. J. et al. (1977) Nucleic Acids. Res., 4, 3065-3081. Konarska, M. M. et al. (1984) Cell, 38, 731-736. Contreas, R. et al. (1982) Nucleic Acids. Res., 10, 6353-6363. Paterson, B. M. and Rosenberg, M. (1979) Nature, 279, 696-701.