 | | | | 7mG(ppp)A RNA Cap Structure Analog | | | |
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Prices are in US dollars and valid only for US orders.
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Description:
The 5' terminal m7G cap present on most eukaryotic mRNAs promotes translation in vitro at the initiation level (1,2,3). For most RNAs, elimination of the cap structure causes a loss of stability, especially against exonuclease degradation (4), and a decrease in the formation of the initiation complex of mRNAs for protein synthesis (4,5). Certain prokaryotic mRNAs containing a 5´ terminal cap structure are translated as efficiently as or more efficiently than eurkaryotic mRNAs in a euraryotic cell-free protein synthesizing system (5). Also a cap requirement has been observed for splicing eurkaryotic substrate RNAs (6).
A method using E. coli RNA Polymerase primed with m7G(5´)ppp(5´)G or m7G(5´)ppp(5´)A for an efficient in vitro synthesis of capped RNAs has been developed by Contreas (7). Larger amounts of capped RNAs are produced by transcription systems using SP6 RNA polymerase primed with m7G(5´)ppp(5´)G (6).
Sequence: 7mG(5')ppp(5')A
Properties & Usage
Supplied As: Sodium Salt
Suggested Working Concentration: 10 mM
Recommended Resuspension Volume: 110 μl
Molecular Weight:
Theoretical: 787 daltons
Storage Conditions
Storage Temperature:
-20°C
Notes
General notes:- Addition of 110 µl water gives approximately a 10 mM solution.
References
- Shatkin, A.J. (1978) Cell, 9, 645-653.
- Fillipowicz, W. (1978) FEBS Lett., 96, 1-11.
- Banerjee, A.K. (1980) Microbiol. Rev., 44, 175-205.
- Miura, K. (1981) Adv. Biophys., 14, 205-238.
- Shatkin, A.J. et al. (1977) Nucl. Acids Res., 4, 3065-3081.
- Konarska, M.M. et al. (1984) Cell, 38, 731-736.
- Contreas, R. et al. (1982) Nucl. Acids Res., 10, 6353-6363.
- Paterson, B.M. and Rosenberg, M. (1979) Nature, 279, 696-701.
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