 | | | | 7mG(ppp)G RNA Cap Structure Analog | | | |
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Prices are in US dollars and valid only for US orders.
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Description:
The 5´ terminal m7G cap present on most eukaryotic mRNAs promotes translation, in vitro, at the initiation level (1). For most RNAs, the cap structure increases stability, decreases susceptibility to exonuclease degradation (2), and promotes the formation of mRNA initiation complexes (2,3). Certain prokaryotic mRNAs with 5´ terminal cap structures are translated as efficiently as eukaryotic mRNAs in a eukaryotic cell-free protein synthesizing system (3). Splicing of certain eukaryotic substrate RNAs has also been observed to require a cap structure (4).
Priming with m7G(5´ )ppp(5´ )G (#S1404S) helps produce larger amounts of capped RNA in transcription systems using SP6 RNA polymerase (4), T7 RNA polymerase (5), and T3 RNA polymerase.
A method for efficient in vitro synthesis of capped RNA using E.coli RNA polymerase primed with m7G(5´ )ppp(5´ )G (#S1404S) or m7G(5´ )ppp(5´ )A (#S1405S) has been developed by Contreas et al. (6).
Sequence: 7mG(5´)ppp(5´)G
Properties & Usage
Supplied As: Sodium Salt
Suggested Working Concentration: 10 mM
Recommended Resuspension Volume: 131 μl
Molecular Weight:
Theoretical: 803 daltons
Storage Conditions
Storage Temperature:
-20°C
Notes
General notes:- Addition of 131 μl water gives approximately a 10 mM solution.
References
- Shatkin, A.J. (1978) Cell, 9, 645-653.
- Fillipowicz, W. (1978) FEBS Lett., 96, 1-11.
- Banerjee, A.K. (1980) Microbiol. Rev., 44, 175-205.
- Miura, K. (1981) Adv. Biophys., 14, 205-238.
- Shatkin, A.J. et al. (1977) Nucl. Acids Res., 4, 3065-3081.
- Konarska, M.M. et al. (1984) Cell, 38, 731-736.
- Contreas, R. et al. (1982) Nucl. Acids Res., 10, 6353-6363.
- Paterson, B.M. and Rosenberg, M. (1979) Nature, 279, 696-701.
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