 |  |  | | M13/pUC Sequencing Primer (-47) (24-mer) |  | |  |
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Prices are in US dollars and valid only for US orders.
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 Description: This primer is suitable for sequencing in the M13-mp2, mp5, mp7, mp8...19 (1,2), MWJ22(3) and pUC vectors ( i.e. pUC7, pUC8, pUC9...19) (2).
After annealing to M13mp18-lac template DNA the 5´ end of the primer lies 47 nucleotides back from the HindIII restriction site.
Sequence: 5´ d(CGCCAGGGTTTTCCCAGTCACGAC) 3´ Phosphorylated:
No
Length: 24 -mer
Properties & Usage

 Supplied As: Lyophilized Triethylammonium Salt
Suggested Working Concentration: 10 μM Recommended Resuspension Volume: 210 μl
Storage Conditions

 Storage Temperature: -20°C
References


- Heidecker, G., Messing, J. and Groneborn, B. (1980) Gene, 10, 69-73.
- Messing, J., Vieira, J., Yanish-Perron, C. (1984) Gene, 33, 102-119.
- Rothstein, R., Lall, L.F., Bahl, C.P., Narang, S.A. and Wu, R. (1979) R. Wu (Eds.), Methods Enzymol., 68, pp. 98-109. New York: Academic Press.
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