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M13/pUC Sequencing Primer (-47) (24-mer)
Catalog # Size Concentration Price Qty  
S1224S 0.5 A260 units   $100.00
Prices are in US dollars and valid only for US orders.
Download:MSDS PDF


Description:
This primer is suitable for sequencing in the M13-mp2, mp5, mp7, mp8...19 (1,2), MWJ22(3) and pUC vectors ( i.e. pUC7, pUC8, pUC9...19) (2).
After annealing to M13mp18-lac template DNA the 5´ end of the primer lies 47 nucleotides back from the HindIII restriction site.

Sequence: 5´ d(CGCCAGGGTTTTCCCAGTCACGAC) 3´ 
Phosphorylated: No
Length: 24 -mer


Properties & Usage


Supplied As: Lyophilized Triethylammonium Salt

Suggested Working Concentration: 10 μM
Recommended Resuspension Volume: 210 μl



Storage Conditions


Storage Temperature:
-20°C


References


  1. Heidecker, G., Messing, J. and Groneborn, B. (1980) Gene, 10, 69-73.
  2. Messing, J., Vieira, J., Yanish-Perron, C. (1984) Gene, 33, 102-119.
  3. Rothstein, R., Lall, L.F., Bahl, C.P., Narang, S.A. and Wu, R. (1979) R. Wu (Eds.), Methods Enzymol., 68, pp. 98-109. New York: Academic Press.

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