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High Fidelity (HF) Restriction Enzyme Sampler |
 | | | | High Fidelity (HF) Restriction Enzyme Sampler |
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This product is not available for online ordering.
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Description:
New England Biolabs provides customers with high quality tools for a wide range of molecular biology applications. As part of our ongoing commitment to the study and improvement of restriction enzymes, we are pleased to introduce a line of restriction enzymes that have been engineered for maximum performance, convenience and flexibility. These engineered enzymes have the same specificity as their established counterparts with the benefit of reduced star activity. In addition, all of these engineered enzymes work optimally in NEBuffer 4, which has the highest level of enzyme compatibility, and will simplify double digest reactions. They are all Time-Saver qualified, and digest substrate DNA in five minutes. In order to distinguish these engineered enzymes, the letters –HF™ have been added to the restriction enzyme name and they are packaged in unique purple-capped tubes.
The Sampler Includes: BamHI-HF™ - 20 μl (400 units)
EcoRI-HF™ - 20 μl (400 units)
SalI-HF™ - 20 μl (400 units)
NotI-HF™ - 20 μl (400 units)
NEBuffer 4 - 1.5 ml (10X)
BSA (100X) Application:
Many techniques such as cloning, genotyping, mutational analysis, mapping, probe preparation, sequencing and methylation detection employ a wide range of reaction conditions and require the use of enzymes under suboptimal conditions. HF™ enzymes have reduced star activity offering increased flexibility to reaction setup and help maximize results under a wide range of conditions.
Kit Components:
BamHI-HF™
BSA
EcoRI-HF™
NEBuffer 4
NotI-HF™
SalI-HF™
FAQs
- How does the level of star activity of BamHI-HF compare to BamHI?
- How does the level of star activity of EcoRI-HF compare to EcoRI?
- How does the level of star activity of SalI-HF compare to SalI?
- How does the level of star activity of NotI-HF compare to NotI?
Reagents Sold Separately
BamHI-HF™
BSA
EcoRI-HF™
NEBuffer 4
NotI-HF™
SalI-HF™
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