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Trypsin-digested BSA MS Standard (CAM-modified) |
 | | | | Trypsin-digested BSA MS Standard (CAM-modified) | | | |
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Prices are in US dollars and valid only for US orders.
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Description:
A complex mixture of peptides produced by Trypsin digestion of Bovine Serum Albumin (BSA) that was reduced and alkylated with Iodoacetamide (CAM modified). This peptide mixture can be used to test a Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight (MALDI-TOF) or Electrospray Ionization (ESI) mass spectrometer (TOF, Q-TOF or Ion Trap).
Useful Range: 500 to 3000 Daltons.
Source:
BSA (GENBANK P02769) was digested using Modified Trypsin (TPCK-treated).
Reaction & Storage Conditions
Storage Temperature:
-20°C
Supplied in lyophilized form.
Notes
Usage notes:- Suggested volume to resuspend: 500 µl. Avoid repeated freeze/thaw cyles once in solution.
Quality Control for Current Lot
Quality control values for a specific lot can be found on the datacard which accompanies each product.
Quality Assurance Statement:
Peptides are free of salts, glycerol and detergents.
NanoLC-ESI MS/MS:
One hundred fmol of the peptide mixture was subjected to nano-reverse-phase liquid chromatography on an Agilent NanoLC C18/Chip 6330 Ion Trap and developed with a water to acetonitrile gradient with both solvents containing 0.1% formic acid. The MS/MS data were analyzed with Mascot, 250 spectra were selected for analysis and a score of 800 or greater was obtained.
MALDI-TOF MS:
0.1 to 1 µl of each of the peptide mixture (0.5 to 5 pmol) was mixed with 1 µl of α-cyano-4-hydroxycinnamic acid matrix solution, air-dried and subjected to MALDI-TOF MS analysis on a Waters Micro MX MALDI-TOF MS. The spectra obtained contains more than 15 resolved peaks which match the theoretical peaks.
MALDI Analysis of BSA Digest: The BSA digest solution was diluted to 1 pmol/μl and mixed at a 1:1 ratio with α-cyano-4-hydroxycinnamic acid (10 mg/μl in 50:50 acetonitrile:water with 0.1% trifluoroacetic acid). Two μl of the digest/matrix solution were then spotted directly onto a MALDI target plate. The sample was then analyzed on a MALDI micro MX Mass Spectrometer (Waters Corporation) with a laser energy of 150.
Online Analysis of BSA Digest: The BSA digest solution was diluted to 100 fmol/μl with 0.1% formic acid. One μl of the digest solution was then injected into an HPLC-Chip Cube system and separated on a Protein ID chip packed with Zorbax 300SB-C18 5 μm material (Agilent Technologies). Peptides were separated using a 40 min 5-45% B linear gradient (A = 0.1% formic acid, B = CH3CN, 0.1% formic acid) at a flow rate of 0.5 μl/min and analyzed online by a 6330 Ion Trap Mass Spectrometer with a nano-electrospray ionization source (Agilent Technologies). The acquisition range was from 300 to 1800 m/z and a capillary voltage of 1800 V was used.
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New
England Biolabs, Inc. is an ISO 9001 and ISO
14001 Certified Company.
NEB certifies that it is a small business in accordance with the US Small Business Administration and 13 CFR 121.201 |
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