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FAQs for Protein Tools Technical Reference for Protein Tools Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder PCR Selection Tool Isoschizomers DNA Sequences and Maps REBASE Enterokinase, light chain Factor Xa Protease Genenase I Proteinase K

Home > Products > Protein Tools > Proteases > Furin Furin Catalog # Size Concentration Price Qty   P8077L 250 units 2,000 units/ml $460.00 P8077S 50 units 2,000 units/ml $115.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Substrate Specificity: Description: Furin is a ubiquitous subtilisin-like proprotein convertase. It is the major processing enzyme of the secretory pathway and is localized in the trans-golgi network (1,2). Substrates of Furin include blood clotting factors, serum proteins and growth factor receptors such as the insulin-like growth factor receptor (3). The minimal cleavage site is Arg-X-X-Arg'. However, the enzyme prefers the site Arg-X-(Lys/Arg)-Arg'. An additional arginine at the P6 position appears to enhance cleavage (4). Furin is inhibited by EGTA, α1- Antitrypsin Portland (5) and polyarginine compounds (6). Source: Isolated from Spodoptera frugiperda (Sf9) cells infected with recombinant baculovirus carrying truncated human furin (kindly provided by R. Fuller) (3). Enzyme Properties Specificity: The minimal cleavage site is Arg-X-X-Arg. However, the enzyme prefers the site Arg-X-(Lys/Arg)-Arg. Molecular Weight: Theoretical: 52.7 kDa and Apparent: 57 kDa Reaction & Storage Conditions Unit Definition: One unit is defined as the amount of Furin that will release 1 pmol of AMC from the fluorogenic peptide BOC-RVRR-AMC (Bachem #I-1645) in one minute (1 pmol of AMC/min) at 30°C. Unit Assay Conditions: 100 mM HEPES (pH 7.5 @ 25°C), 0.5% Triton X-100, 1 mM CaCl2, 1 mM 2-mercaptoethanol, 100 µM B0C-RVRR-AMC and enzyme in a 100 µl volume at 30°C. Fusion Protein Digestion: One unit will cut 25 μg test substrate to 95% completion in 6 hours or less, while 0.5 units will cut 25 μg of test substrate to 95% completion in 16 hours or less. Fusion Protein Digestion Conditions: Furin is added to 25 μg of an MBP fusion protein testsubstrate, MBP-ΔSal. The reaction is carried out in 25 ul, 100 mM Hepes (pH 7.5 @25°C), 0.5%Triton X-100, 1mM CaCl2, 1mM 2-mercaptoethanol at 25°C Concentration: 2,000 units/ml Storage Conditions: 10 mM MES 1 mM CaCl2 50% Glycerol pH 7.0 @ 25°C Storage Temperature: -20°C Notes Usage notes: Both Furin and Onchocerca volvulus Blisterase will cleave peptide substrates with the sequence, Arg-X- (Lys/Arg)-Arg. However, the ability of either enzyme to cleave a particular protein substrate depends on its tertiary structure as well as on the amino acids immediately surrounding the cleavage site (7). References van den Ouweland, A.M.W. et al. (1990) Nucl. Acids Res., 18, 664. Steiner, D.F. (1998) Curr. Opin. Chem. Biol., 2, 31-39. Bravo, D.A. et al. (1994) J. Biol. Chem., 269, 25830-25837. Krysan, D.J. et al. (1999) J. Biol. Chem., 274, 23229-23234. Jean, F. et al. (1998) Proc. Natl. Acad. Sci. USA, 95, 7293-7298. Cameron, A. et al. (2000) J. Biol. Chem., 275, 36741-36749. Poole, C.B. et al. (2003) J. Biol. Chem., 278, 36183-36190. Companion Products Enterokinase, light chain Factor Xa Protease Genenase I Proteinase K New England Biolabs, Inc. is an ISO 9001 and ISO 14001 Certified Company. NEB certifies that it is a small business in accordance with the US Small Business Administration and 13 CFR 121.201