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FAQs for Protein Tools Technical Reference for Protein Tools Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE Enterokinase, light chain Furin Genenase I Proteinase K

Home > Products > Protein Tools > Proteases > Factor Xa Protease Factor Xa Protease Catalog # Size Concentration Price Qty   P8010L 250 μg 1 mg/ml $224.00 P8010S 50 μg 1 mg/ml $56.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Substrate Specificity: Description: Factor Xa cleaves after the arginine residue in its preferred cleavage site Ile-(Glu or Asp)-Gly-Arg. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate (1,2,3). The most common secondary site, among those that have been sequenced, is Gly-Arg. There seems to be a correlation between proteins that are unstable in E.coli and those that are cleaved by Factor Xa at secondary sites; this may indicate that these proteins are in a partially unfolded state (Walker, I., Riggs, P., unpublished observations). Factor Xa will not cleave a site followed by proline or arginine. Source: Factor Xa Protease is purified from bovine plasma and activated by treatment with the activating enzyme from Russell's viper venom. Enzyme Properties Deactivation: Dansyl-glu-gly-arg-chloromethyl ketone (CALBIOCHEM, #251700) will irreversibly inactivate Factor Xa by covalent attachment at the active site. In a reaction containing 20 μg/ml Factor Xa, 2 μM dansyl-glu-gly-arg-chloromethyl ketone will inactivate >95% of the Factor Xa in 1 minute at room temperature. Molecular Weight: Theoretical: 43 kDa and Apparent: 50 kDa Reaction & Storage Conditions Unit Definition: 1 μg of Factor Xa will cleave 50 μg of MBP fusion protein test substrate, MBP-ΔSal to 95% completion in a total reaction volume of 50 μl in 6 hours or less at 23°C in 20 mM Tris-HCl (pH 8.0 @ 25°C) with 100 mM NaCl and 2 mM CaCl2. Concentration: 1 mg/ml Storage Conditions: 20 mM HEPES 500 mM NaCl 2 mM CaCl2 50% Glycerol pH 8.0 @ 25°C Storage Temperature: -20°C Notes General notes: The test substrate MBP-ΔSal is maltose-binding protein fused to a truncated form of paramyosin, with the amino acids Ile-Glu-Gly-Arg at the fusion joint. Greater than 95% of the fusion protein is cleaved in 6 hours or less. References Nagai, K. et al. (1985) Proc. Natl. Acad. Sci. USA, 82, 7252-7255. Quinlan, R.A. et al. (1989) J. Cell Sci., 93, 71-83. Eaton, D. et al. (1986) Biochemistry, 25, 505-512. Companion Products Enterokinase, light chain Furin Genenase I Proteinase K