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 Description: RNase B is a high mannose glycoprotein (1-3) that can be used as a positive control for endoglycosidases that cleave N-linked carbohydrates. RNase B has a single N-linked glycosylation site which makes it ideal for SDS-PAGE gel shift assays.
Source: Bovine pancreas
Properties & Usage

 Molecular Weight: Theoretical: 17,000 daltons
Reaction & Storage Conditions

 Concentration: 5 mg/ml
Storage Conditions: 50 µl Milli-Q water
Storage Temperature: -20°C
Notes

 General notes:- 250 μg is enough for approximately 20 reactions.
FAQs


- Do detergents inhibit exoglycosidases/endoglycosidases?
References


- Plummer, T.H. and Hirs, C. (1964) J. Biol. Chem., 239, 2530.
- Plummer, T.H. Jr., Tarentino, A. and Maley, F. (1968) J. Biochem. (Tokyo), 243, 5185.
- Liang, C.-J. Yamashita, K. and Kobata, A. (1980) J. Biolchem (Tokyo), 88, 51.
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