New England Biolabs
To access your account, log in or register.
Products Technical Reference Customer Service My NEB Account
Contact NEB About Us Site Map Request a Catalog OEM at NEB International Orders Freezer Program Quick Order
Related Information
FAQs for RNase B
FAQs for Protein Tools
Technical Reference for Protein Tools
Favorite Tools
Enzyme Finder
NEBcutter
NEBuffer Chart
Double Digest Finder
Isoschizomers
DNA Sequences
and Maps
REBASE
Related Products
Companion Products
Endo H
Endo Hf
PNGase F
PNGase F (Glycerol Free)
RNase B
Catalog # Size Concentration Price Qty  
P7817S 250 μg 5 mg/ml $56.00
Prices are in US dollars and valid only for US orders.
Download:MSDS PDF


Description:
RNase B is a high mannose glycoprotein (1-3) that can be used as a positive control for endoglycosidases that cleave N-linked carbohydrates. RNase B has a single N-linked glycosylation site which makes it ideal for SDS-PAGE gel shift assays.

Source:
Bovine pancreas


Properties & Usage


Molecular Weight:
Theoretical: 17,000 daltons


Reaction & Storage Conditions


Concentration:
5 mg/ml

Storage Conditions:
50 µl Milli-Q water


Storage Temperature:
-20°C


Notes


General notes:
  1. 250 μg is enough for approximately 20 reactions.

FAQs


  1. Do detergents inhibit exoglycosidases/endoglycosidases?

References


  1. Plummer, T.H. and Hirs, C. (1964) J. Biol. Chem., 239, 2530.
  2. Plummer, T.H. Jr., Tarentino, A. and Maley, F. (1968) J. Biochem. (Tokyo), 243, 5185.
  3. Liang, C.-J. Yamashita, K. and Kobata, A. (1980) J. Biolchem (Tokyo), 88, 51.


Companion Products


Endo H
Endo Hf
PNGase F
PNGase F (Glycerol Free)

Privacy, Limitations, Warranty, Disclaimer, Copyright & Trademark