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ColorPlus Prestained Protein Marker, Broad Range (7-175 kDa) |
 | | | | ColorPlus Prestained Protein Marker, Broad Range (7-175 kDa) | | | |
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Prices are in US dollars and valid only for US orders.
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Description:
ColorPlus Prestained Protein Marker, Broad Range includes a mixture of purified proteins covalently coupled to colored dye that resolves to 8 bands of even intensity, including one orange and one green reference band for easy orientation when electrophoresed (1). The covalent coupling of the dye to the proteins affects their electrophoretic behavior in SDS-PAGE gels relative to unstained proteins (1). For precise molecular weight determinations, use NEB's unstained Protein Marker, Broad Range (NEB #P7702) or Protein Ladder (NEB #P7703) in addition to the prestained marker.
Contents: 0.1-0.2 mg/ml of each protein in 50 mM NaCl, 63.5 mM Tris-HCl (pH 7.0 @ 25°C), 1 mM Na2EDTA, 2% (w/v) SDS, 40 mM DTT, 0.01% (w/v) phenol red and 10% glycerol.
10-20% SDS PAGE. This marker was recently recalibrated using the Protein Ladder (NEB #P7703), and the revised apparent molecular weights are shown. The formulation of the marker is unchanged.
Effective Size Range: 7 kDa to 175 kDa
Source:
E. coli
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| MBP-β-galactosidase* | | E. coli | | 175 | | |
| MBP-truncated-β-galactosidase* | | E. coli | | 80 | | |
| MBP-CBD | | E. coli | | 58 | | |
| CBD-Mxe Intein-2CBD | | E. coli | | 46 | | |
| CBD-Mxe Intein | | E. coli | | 30 | | |
| CBD-E. coli Par | | E. coli | | 23 | | |
| Lysozyme | | chicken egg white | | 17 | | |
| Aprotinin | | bovine lung | | 7 | | |
Recommended Load: 15 μl
Storage Conditions
Storage Conditions:
63.5 mM Tris-HCl
50 mM NaCl
40 mM DTT
1 mM Na2EDTA
10% Glycerol
0.01% Phenol Red
2% SDS (w/v)
pH 7.0 @ 25°C
Storage Temperature:
-20°C
Notes
General notes:- * MBP = maltose-binding protein. MBP-β-galactosidase = fusion of MBP and β-galactosidase. MBP-truncated-β-galactosidase = fusion of MBP and a truncated β-galactosidase. MBP-CBD = fusion of MBP and chitin binding domain. CBD-Mxe Intein= fusion of the chitin binding domain followed by the Mxe Intein. CBD-Mxe Intein-2CBD= fusion of chitin binding domain and the Mxe Intein followed by 2 copies of the chitin binding domain. CBD-E. coli Par= fusion of the chitin binding domain followed by an E. coli parvulin-like protein.
- Apparent molecular weights of every band are determined on Invitrogen Novex 10-20% Tris-glycine SDS PAGE gels using NEB's Protein Ladder (NEB #P7703)..
- To maximize shelf life, marker should be boiled upon receipt and aliquotted into single-use tubes. Store at -20°C.
Usage notes:- Recommended load for SDS-PAGE Analysis is 15 μl, while 6 μl is recommended for Western blot analysis.
FAQs
- Is the ColorPlus Prestained Protein Marker stable at room temperature?
- After repeated freeze/thaws, I have noticed a fading or speading out of the low molecular weight bands in the ColorPlus Prestained Protein Marker. Is there any way to extend the shelf-life of this product?
- Why do the apparent molecular weight values for the prestained protein markers appear to be different in different gel types?
Protocols
Protocols for ColorPlus Prestained Protein Marker, Broad Range (7-175 kDa)
Note: Apparent molecular weight values for prestained protein markers can be different when run on different gel types.
References
- Laemmli, U.K. (1970) Nature, 227, 680.
- Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, (2nd ed.), Cold Spring Harbor Laboratory Press.
Companion Products
Prestained Protein Marker, Broad Range (7-175 kDa)
Protein Ladder (10-250 kDa)
Protein Marker, Broad Range (2-212 kDa)
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New
England Biolabs, Inc. is an ISO 9001 and ISO
14001 Certified Company.
NEB certifies that it is a small business in accordance with the US Small Business Administration and 13 CFR 121.201 |
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