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Prestained Protein Marker, Broad Range (7-175 kDa) |
 | | | | Prestained Protein Marker, Broad Range (7-175 kDa) | | | |
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Prices are in US dollars and valid only for US orders.
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Description:
Prestained Protein Marker, Broad Range is a mixture of purified proteins covalently coupled to a blue dye that resolves to 8 bands when electrophoresed. The protein concentrations are carefully balanced for even intensity. The covalent coupling of the dye to the proteins affects their electrophoretic behavior in SDS-PAGE gels relative to unstained proteins (1). The apparent molecular weight of the prestained proteins are given in the table below. For precise molecular weight determinations, use NEB's unstained Protein Marker, Broad Range (NEB #P7702) or Protein Ladder (NEB #P7703) in addition to the prestained marker.
Contents: 0.1–0.2 mg/ml of each protein in 70 mM Tris-HCl (pH 6.8 @ 25°C), 33 mM NaCl, 1 mM Na2EDTA, 2% (w/v) SDS, 40 mM DTT, 0.01% (w/v) phenol red and 10% glycerol.
10-20% SDS PAGE. This marker was recently recalibrated using the Protein Ladder (NEB #P7703), and the revised apparent molecular weights are shown. The formulation of the marker is unchanged.
Effective Size Range: 7 kDa to 175 kDa
Source:
E. coli
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| MBP-β-galactosidase * | | E. coli | | 175 | | |
| MBP-paramyosin * | | E. coli | | 80 | | |
| MBP-CBD | | E. coli | | 58 | | |
| CBD-Mxe Intein-2CBD | | E. coli | | 46 | | |
| CBD-Mxe Intein | | E. coli | | 30 | | |
| CBD-BmFKBP13 | | E. coli | | 25 | | |
| Lysozyme | | chicken egg white | | 17 | | |
| Aprotinin | | bovine lung | | 7 | | |
Concentration: 0.1 mg/ml to 0.2 mg/ml
Recommended Load: 15 μl
Storage Conditions
Storage Conditions:
70 mM Tris-HCl
33 mM NaCl
40 mM DTT
1 mM EDTA
10% Glycerol
0.01% Phenol Red
2% SDS (w/v)
pH 6.8 @ 25°C
Storage Temperature:
-20°C
Notes
General notes:- Apparent molecular weights of every lot are determined on Invitrogen Novex 10-20% Tris-glycine SDS PAGE gels using NEB's Protein Ladder (NEB #P7703).
- MBP = maltose-binding protein. MBP-β-galactosidase = fusion of MBP and β-galactosidase. MBP-paramyosin = fusion of MBP and paramyosin. MBP-CBD = fusion of MBP and chitin binding domain.
- * MBP = maltose-binding protein. MBP-β-galactosidase = fusion of MBP and β-galactosidase. MBP-paramyosin = fusion of MBP and paramyosin. MBP-CBD = fusion of MBP and chitin binding domain.
- CBD-Mxe Intein=fusion of chitin binding domain and the Mxe Intein
- CBD-Mxe Intein-2CBD=fusion of the chitin binding domain and the Mxe Intein followed by two chitin binding domains
Usage notes:- Recommended load for SDS-PAGE is 15 μl, while 6 μl is recommended for Western blot analysis.
FAQs
- Why do the apparent molecular weight values for the prestained protein markers appear to be different in different gel types?
Protocols
Protocols for Prestained Protein Marker, Broad Range (7-175 kDa)
References
- Laemmli, U.K. (1970) Nature, 227, 680.
- Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, (2nd ed.), X.
Companion Products
ColorPlus Prestained Protein Marker, Broad Range (7-175 kDa)
Protein Ladder (10-250 kDa)
Protein Marker, Broad Range (2-212 kDa)
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New
England Biolabs, Inc. is an ISO 9001 and ISO
14001 Certified Company.
NEB certifies that it is a small business in accordance with the US Small Business Administration and 13 CFR 121.201 |
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