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Protein Ladder (10-250 kDa) |
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Prices are in US dollars and valid only for US orders.
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Description:
Protein Ladder is a mixture of 12 recombinant, highly purified proteins, which resolve into clearly identifiable sharp bands from 10-250 kDa when analyzed by SDS-PAGE and stained with Coomassie Brilliant Blue R-250 (1). This ladder is intended for use as a precise size standard when performing SDS-PAGE to calculate the molecular weight of a protein of interest. The 25 and 80 kDa bands have triple the intensity of the other proteins and serve as reference indicators. The molecular weights of the Protein Ladder are confirmed by sequencing, mass spectrometry and migration in a Laemmli SDS-Page Tris-Glycine system (3).
10-20%
SDS PAGE
Effective Size Range: 10 kDa to 250 kDa
Concentration: 0.1 mg/ml to 0.3 mg/ml
Recommended Load: 5 μl
Storage Conditions
Storage Conditions:
70 mM Tris-HCl
33 mM NaCl
40 mM DTT
1 mM Na2EDTA
10% Glycerol
0.01% Bromophenol Blue
2% SDS (w/v)
pH 6.8 @ 25°C
Storage Temperature:
-20°C
Notes
Usage notes:- Thaw the Protein Ladder at room temperature.
- Vortex gently to ensure the solution is homogeneous.
- For silver stained gels, dilute the Protein Ladder in 1:15 in buffer (70 mM Tris-HCl, pH 6.8, 33 mM NaCl, 1 mM Na2EDTA, 2% SDS, 40 mM DTT, 0.01% Bromophenol Blue and 10% glycerol).
FAQs
- Can I label the Protein Ladder with horseradish peroxidase (HRP) for western blotting?
References
- Laemmli, U.K. (1970) Nature, 227, 680.
- Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, (2nd ed.), x.
- Ma, D. and Xu, MQ., New England Biolabs, unpublished observations.
Companion Products
ColorPlus Prestained Protein Marker, Broad Range (7-175 kDa)
Prestained Protein Marker, Broad Range (7-175 kDa)
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