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Protocols for Protein Marker, Broad Range (2-212 kDa) FAQs for Protein Tools Technical Reference for Protein Tools Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE ColorPlus Prestained Protein Marker, Broad Range (7-175 kDa) Prestained Protein Marker, Broad Range (7-175 kDa)

Home > Products > Protein Tools > Protein Molecular Weight Markers > Protein Marker, Broad Range (2-212 kDa) Protein Marker, Broad Range (2-212 kDa) Catalog # Size Concentration Price Qty   P7702L 750 mini-gel lanes   $264.00 P7702S 150 mini-gel lanes   $66.00 Prices are in US dollars and valid only for US orders. Download: Technical Bulletin|MSDS PDF Description: Protein Marker, Broad Range is a mixture of purified proteins with known amino acid sequences. They are resolved to 13 sharp bands when analyzed by SDS-PAGE (Tris-Glycine) and stained with Coomassie Blue R-250 (1). Two bands (BSA, MW 66.4 kDa and Triosephosphate isomerase, MW 26.6 kDa) are at double intensity to serve as reference points. 10-20% SDS-PAGE Insulin chains are unresolved by SDS-PAGE (Tris-Glycine). Effective Size Range: 2 kDa to 212 kDa Protein Source Molecular Weight (daltons) Myosin rabbit muscle 212,000 MBP-β-galactosidase E. coli 158,194 β-Galactosidase E. coli 116,351 Phosphorylase b rabbit muscle 97,184 Serum albumin bovine 66,409 Glutamic dehydrogenase bovine liver 55,561 MBP2* E. coli 42,710 Thioredoxin reductase E. coli 34,622 Triosephosphate isomerase E. coli 26,972 Trypsin inhibitor soybean 20,100 Lysozyme chicken egg white 14,313 Aprotinin bovine lung 6,517 Insulin A bovine pancreas 3,400 B chain bovine pancreas 2,340 Concentration: 0.1 mg/ml to 0.2 mg/ml Recommended Load: 6 μl Storage Conditions Storage Conditions: 70 mM Tris-HCl 33 mM NaCl 40 mM Dithiothreitol 1 mM EDTA 10% Glycerol 0.01% Bromophenol Blue 2% SDS (w/v) pH 6.8 @ 25°C Storage Temperature: -20°C Notes General notes: MBP2*= maltose-binding protein. MBP-β-galactosidase = fusion of MBP and β-galactosidase. Trypsin inhibitor (soybean) is a mixture of three isoforms: A-20,094 Da; B-20,040 Da; C-20,167 Da. These isoforms migrate as one unresolved band using Tris-Glycine gels and as a doublet using Tris-Tricine gel. [Schagger, H. and Von Jagow, G. (1987) Anal. Biochem. 166, 368-379]. Apparent molecular weights of every lot are determined on 10-20% gradient gels using NEB's Protein Ladder (NEB #P7703). References Laemmli, U.K. (1970) Nature, 227, 680. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, (2nd ed.), X. Companion Products ColorPlus Prestained Protein Marker, Broad Range (7-175 kDa) Prestained Protein Marker, Broad Range (7-175 kDa)