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Protocols for Bio-P1 Peptide FAQs for Protein Tools Technical Reference for Protein Tools Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder PCR Selection Tool Isoschizomers DNA Sequences and Maps REBASE Flu-P1 Peptide IMPACT-CN System IMPACT-TWIN System Peptide-Carrier™ Kit

Home > Products > Protein Tools > Protein Labeling > Bio-P1 Peptide Bio-P1 Peptide Catalog # Size Concentration Price Qty   P6607S 0.29 mg   $105.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: Bio-P1 peptide is a synthetic peptide containing an N-terminal cysteine and a biotinylated lysine. This peptide can be used in conjunction with the IMPACT Kit (NEB #E6901) to site specifically incorporate a biotin moiety into a bacterially-expressed protein. The Bio-P1 peptide ligates to the C-terminus of proteins containing a C-terminal thioester (1,2,3). This procedure is termed intein-mediated protein ligation (IPL). The thioester-tagged proteins are purified using the IMPACT Kit in which, preferably, 2-mercaptoethanesulfonic acid (MESNA) was used to induce intein cleavage. This peptide can also be used in conjunction with the Peptide Carrier Kit (NEB# E6600) to label Carrier Protein 39 and 27 for western blot analysis.  The peptide sequence is NH2-CDPEK(Bt)DS-CONH2. The K(Bt) represents a lysine with a biotin covalently attached to its ε-amino group. There is a 7 carbon linker between the biotin and the ε-amino group of the lysine. Source: An HPLC purified synthetic peptide Applications: Site specifically labeling the C-terminus of a protein with biotin Reagents Supplied: IPL Reaction Buffer (10X) Properties & Usage Molecular Weight: Theoretical: 1,132 daltons Reaction & Storage Conditions Reaction Conditions: 1X IPL Reaction Buffer Incubate at 25°C. 1X IPL Reaction Buffer: 100 mM Tris-HCl 250 mM NaCl 10 mM MESNA pH 8.5 @ 25°C Storage Temperature: -20°C Notes Usage notes: Upon first use resuspend the peptide in 50 µl of 5X IPL Reaction Buffer. Separate into 10 µl aliquots and store at -70°C for future use. Protocols Protocols for Bio-P1 Peptide Figure 1: Intein-mediated Protein Ligation References Evans, T. C. et al. (1998) Protein Sci., 2254-2264. Muir, T. W. (1998) Proc. Natl. Acad. Sci. USA, 95, 6705-6710. Xu, M.-Q and Evans, T. C., Jr. (2001) Methods, 24, 257-277. Companion Products Flu-P1 Peptide Peptide-Carrier™ Kit New England Biolabs, Inc. is an ISO 9001 and ISO 14001 Certified Company. NEB certifies that it is a small business in accordance with the US Small Business Administration and 13 CFR 121.201