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Autocamtide-2 Related Inhibitory Peptide (AIP) |
 | | | | Autocamtide-2 Related Inhibitory Peptide (AIP) | | | |
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Prices are in US dollars and valid only for US orders.
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Description:
This synthetic peptide, Autocamtide-2 Related Inhibitory Peptide (AIP), is a highly specific and potent inhibitor of Ca2+/Calmodulin-dependent Protein Kinase II (CaMKII). AIP is a non-phosphorylatable analog of Autocamtide-2, a highly selective peptide substrate for CaMKII (NEB #P6062). AIP inhibits CaMKII with IC50* of 100 nm. The AIP inhibition is non-competitive with respect to Syntide-2 (CaMKII Peptide Substrate, NEB #P6059) and ATP but competitive with respect to Autocamtide-2. The inhibition is unaffected by the presence or absence of Ca2+/calmodulin. 1 µM AIP completely inhibits CaMKII activity, but did not affect PKA, PKC and CaMKIV (1).
* IC50 is a concentration of the inhibitor required to obtain 50% inhibition.
Amino Acid Sequence:
KKALRRQEAVDAL (Lys-Lys-Ala-Leu-Arg-Arg-Gln-Glu-Ala-Val-Asp-Ala-Leu)
Purity: AIP is > 95% pure as determined by HPLC and mass spectrometry.
Suggested Working Concentration:Dilution of the supplied solution (10 mg/ml = 6.7 mM) to a final concentration of 1 µM is recommended.
Enzyme Properties
Protein Kinase Substrate Recognition
Molecular Weight:
Theoretical: 1,498 daltons
Reaction & Storage Conditions
Concentration:
10 mg/ml
Storage Conditions:
1 X Sterile purified water
Storage Temperature:
-20°C
Notes
General notes:- Syntide-2 is a recommended substrate to study the inhibition of CaMKII with AIP, which is noncompetitive with Syntide-2 (1)
References
- Ishida, A. et al. (1995) Biochem. Biophys. Res. Commun., 212, 806-812.
Companion Products
Autocamtide-2 Peptide Substrate
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