To access your account, log in or register.	shopping cart | log in

FAQs for Protein Tools Technical Reference for Protein Tools Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE

Home > Products > Protein Tools > Protein Kinases/Substrates/Inhibitors > Syntide-2 (CaMKII Peptide Substrate) Syntide-2 (CaMKII Peptide Substrate) Catalog # Size Concentration Price Qty   P6059L 2.5 mg 10 mg/ml $300.00 P6059S 0.5 mg 10 mg/ml $75.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: Syntide-2 is a synthetic peptide substrate for Ca2+/Calmodulin-dependent Protein Kinase II (CaMKII) derived from the phosphorylation site 2 in glycogen synthase (1, 2). The presence of basic residues makes it suitable for kinase assay using the phosphocellulose paper binding method (3). CaMKII phosphorylates Syntide-2 with a Km of 12 μM (1).  Amino Acid Sequence:  PLARTLSVAGLPGKK (Pro-Leu-Ala-Arg-Thr-Leu-Ser-Val-Ala-Gly-Leu-Pro-Gly-Lys-Lys) Purity: Syntide-2 is > 95% pure as determined by HPLC and mass spectrometry.  Suggested Working Concentration:Dilution of the supplied solution (10 mg/ml = 6.6 mM) to a final concentration of 100 µM is recommended. Enzyme Properties Protein Kinase Substrate Recognition Molecular Weight: Theoretical: 1,508 daltons Reaction & Storage Conditions Concentration: 10 mg/ml Storage Conditions: 1 X Sterile purified water Storage Temperature: -20°C Notes General notes: Syntide-2 is a recommended substrate to study the inhibition of CaMKII with AIP (Autocamtide-2-related Inhibitory Peptide, NEB #P6063), which is noncompetitive with Syntide-2 (2). References Hashimoto, Y. and Soderling T. R. (1987) Arch. Biochem. Biophys., 252, 418-425. Ishida, A. et al. (1995) Biochem. Biophys. Res. Commun., 212, 806-812. Stewart, A. A. (1993) Protein Phosphorylation: A laboratory Manual, In D. G. Hardie et al., (Eds), (pp. 122-127) New York: Oxford University P.