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Abl Peptide Substrate |
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Prices are in US dollars and valid only for US orders.
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Description:
This synthetic peptide is derived from the predicted optimal substrate for c-Abl Protein Tyrosine Kinase (c-Abl) determined by Songyang, et al. (1). The presence of basic lysine residues makes it suitable for enzyme assays using the phosphocellulose-paper binding method (2,3). c-Abl phosphorylates this peptide with a Km of 4 µM(1). Abl Peptide Substrate is suitable for the assay of Abl Protein Kinase (NEB#P6050), which is identical to c-Abl.
Amino Acid Sequence: EAIYAAPFAKKK (Glu-Ala-Ile-Tyr-Ala-Ala-Pro-Phe-Ala-Lys-Lys-Lys).
Supplied in: Milli-Q™ water.
Enzyme Properties
Protein Kinase Substrate Recognition
Reaction & Storage Conditions
Concentration:
10 mg/ml
Storage Temperature:
-20°C
Notes
Usage notes:- Suggested Working Concentration: Dilution of the supplied solution (10 mg/ml = 7.4 mM) to a final concentration of 50-100 µM is recommended.
Quality Control for Current Lot
Quality control values for a specific lot can be found on the datacard which accompanies each product.
Quality Assurance Statement:
Abl Peptide Substrate is > 95% pure as determined by HPLC, and mass spectrometry.
References
- Songyang, Z. et al. (1995) Nature, 373, 536-539.
- Casnellie, J.F. (1991) In T. Hunter and B.M. Sefton (Eds.). Methods in Enzymol., 200, (pp. 115?120). San Diego: Academic Press..
- Yang, Y. and Roach P. J. (1993) In D.G. Hardie (Ed.). Protein Phosphorylation: A Practical Approach, (pp. 122?129). New York: IRL Press..
Companion Products
Abl Protein Tyrosine Kinase (Abl)
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