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CREB Phosphopeptide (GSK-3 Substrate) |
 | | | | CREB Phosphopeptide (GSK-3 Substrate) | | | |
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Description:
This peptide is derived from the Glycogen Synthase Kinase 3 (GSK-3) phosphorylation site at Ser129 of the cAMP Response Element Binding Protein (CREB) (1). Ser11 of the peptide (which corresponds to Ser133 of CREB) has been synthetically phosphorylated, creating a strong determinant for GSK-3 recognition and phosphorylation of Ser7. Ser133 of CREB is thought to be phosphorylated by PKA in vivo, potentiating subsequent GSK-3 phosphorylation. All CREB residue numbers above refer to the 341-amino acid form of CREB, called CREB341 or CREB-B (1).
The presence of basic arginine and lysine residues in the peptide makes it suitable for enzyme assays using the phosphocellulose-paper binding method (2). GSK-3 phosphorylates this phosphopeptide with a Km of 200 µM (3).
Amino Acid Sequence: KRREILSRRPpSYR(Lys-Arg-Arg-Glu-Ile-Leu-Ser-Arg-Arg-Pro-pSer-Tyr-Arg)
Purity: CREB Phosphopeptide is > 90% pure as determined by HPLC and mass spectrometry.
Suggested Working Concentration: Dilution of the supplied solution (10 mg/ml = 5.57 mM) to a final concentration of 400 µM is recommended.
Enzyme Properties
Protein Kinase Substrate Recognition
Molecular Weight:
Theoretical: 1,796 kDa
Reaction & Storage Conditions
Concentration:
10 mg/ml
Storage Conditions:
1 X Sterile purified water
Storage Temperature:
-20°C
References
- Fiol, C. J. et al. (1994) J. Biol. Chem., 269, 32187-32193.
- Yang, Y. and Roach P.J. (1993) In D.G. Hardie (Ed.). Protein Phosphorylation: A Practical Approach, 122-129. New York: IRL Press.
- Wang, Q. M. et al. (1994) J. Biol. Chem., 269, 14566-14574.
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