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Myelin Basic Proteinf (MyBPf) |
 | | | | Myelin Basic Proteinf (MyBPf) | | | |
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Prices are in US dollars and valid only for US orders.
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- Recombinant fusion protein (E. coli)
- General protein kinase substrate
- Suitable for mass spectrometry, Western blot, dot blot and ELISA assays
- No basal phosphorylation
Description:
Myelin Basic Proteinf (MyBPf, isoform 4) is expressed as a 29 kDa fusion protein with a Chitin Binding Domain at its N-terminus, and a 6x His tag at its C-terminus. MyBPf can be used as a substrate for kinases, such as MAP Kinase, cAMP-dependent Protein Kinase, Calmodulin-dependent Protein Kinase, Abl Tyrosine Kinase, etc. (1-4). It has no basal phosphorylation or post-translational modifications. The phosphorylation of MyBPf can be analyzed using a variety of techniques, including western blot, array, dot blot, radioisotope analysis or mass spectrometry. It can also be used in ELISA assays by coating 96-well plates followed by a kinase reaction and phospho-specific antibody detection (Fig. 1).
This pack is suitable for at least one 96-well ELISA.
Reaction Conditions: Reaction can be carried out according to the kinase protocol suggested by the manufacturer.
Figure 1, ELISA Assay: Phosphorylation of recombinant MyBPf and native bovine MyBP by different protein kinases examined by a phospho-specific antibody. Recombinant MyBPf has low background whereas bovine MyBP has high noise to background ratio.
Source:
Isolated from a strain of E. coli that carries a clone expressing the MyBP fusion protein under a T7 expression system.
Properties & Usage
Molecular Weight:
Theoretical: 29 kDa
Reaction & Storage Conditions
Concentration:
0.5 mg/ml
Storage Conditions:
10% Glycerol
2.5 mM acetic acid
Storage Temperature:
-20°C
FAQs
- What is the MyBPf fusion protein sequence?
- What is the protocol for using MyBPf as a Kinase substrate?
- How do I set up a MyBPf -trypsin digest for mass spectrometry analysis?
- How much MyBPf do I coat a 96 well plate for a phosphor-ELISA assay?
- What are the advantages of using MyBPf compared to tissue extracted MyBP?
Quality Control for Current Lot
Quality control values for a specific lot can be found on the datacard which accompanies each product.
Quality Assurance Statement:
MyBPf contains no detectable phosphatase or proteolytic activity. Myelin Basic Protein is ≥ 95% pure as determined by SDS-PAGE analysis.
References
- Carnegie, P. R. et al. (1973) Biochem. J., 135, 569-572.
- Erickson, A. K. et al. (1990) J. Biol. Chem., 265, 19728-19735.
- Kishimoto, A. et al. (1985) J. Biol. Chem., 260, 12492-12499.
- Miyamoto, E. and Kakiuchi, S. (1974) J. Biol. Chem., 249, 2769-2777.
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