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Kemptide (PKA Peptide Substrate)
Catalog # Size Concentration Price Qty  
P6001L 2.5 mg 10 mg/ml $140.00
P6001S 0.5 mg 10 mg/ml $35.00
Prices are in US dollars and valid only for US orders.
Download:MSDS PDF


Description:
Kemptide (PKA Peptide Substrate) is a synthetic peptide substrate for cAMP-dependent protein kinase (PKA) derived from the PKA phosphorylation site in liver pyruvate kinase. The presence of basic arginine residues makes Kemptide suitable for enzyme assays using the phosphocellulose-paper binding method. PKA phosphorylates Kemptide with a Km of 33 µM (1,2).

Amino Acid Sequence: 
LRRASLG(Leu-Arg-Arg-Ala-Ser-Leu-Gly) 

Purity: Kemptide is > 95% pure as determined by HPLC and mass spectrometry. 

Suggested Working Concentration: Dilution of the supplied solution (10 mg/ml = 12.95 mM) to a final concentration of 100-200 µM is recommended.


Enzyme Properties


Protein Kinase Substrate Recognition

Molecular Weight:
Theoretical: 771 daltons


Reaction & Storage Conditions


Concentration:
10 mg/ml

Storage Conditions:
1 X Sterile purified water


Storage Temperature:
-20°C


References


  1. Moore, M.J. et al. (2003) J. Biol. Chem., 278, 10613-10618.
  2. ang, Y. and Roach P.J. (1993) In D.G. Hardie (Ed.). Protein Phosphorylation: A Practical Approach, 122-129. New York: IRL Press.

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