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Sodium Fluoride (Fluoride)
Catalog # Size Concentration Price Qty  
P0759L 5 ml 500 mM $80.00
P0759S 1 ml 500 mM $20.00
Prices are in US dollars and valid only for US orders.
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Description:
Sodium Fluoride (Fluoride, NaF) is a commonly used general inhibitor for protein phosphoseryl and phosphothreonyl phosphatases (PSPs). Routinely Fluoride, combined with Sodium Orthovanadate (NEB# P0758), a general inhibitor for protein phosphotyrosyl phosphatases (PTPs), is used to preserve the protein phosphorylation state in cells, cell lysates, and protein kinase (PK) assays. These inhibitors should ideally be present in all buffers used in the purification of PKs (1, 2). 

Purity: >99% pure 

Suggested Working Concentration: 5-50 mM


Enzyme Properties


Molecular Weight:
Theoretical: 41.99 daltons


Reaction & Storage Conditions


Concentration:
500 mM

Storage Conditions:
1 X Sterile purified water


Storage Temperature:
-20°C


References


  1. Hardie, D.G. et al. (1993) In D. G. Hardie (Ed.). Protein Phosphorylation: A Practical Approach, 62. New York: IRL Press.
  2. Gordon, J.A. (1991) Methods in Enzymology, 201, 477-482.

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