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Sodium Fluoride (Fluoride) |
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 Description: Sodium Fluoride (Fluoride, NaF) is a commonly used general inhibitor for protein phosphoseryl and phosphothreonyl phosphatases (PSPs). Routinely Fluoride, combined with Sodium Orthovanadate (NEB# P0758), a general inhibitor for protein phosphotyrosyl phosphatases (PTPs), is used to preserve the protein phosphorylation state in cells, cell lysates, and protein kinase (PK) assays. These inhibitors should ideally be present in all buffers used in the purification of PKs (1, 2).
Purity: >99% pure
Suggested Working Concentration: 5-50 mM
Enzyme Properties

 Molecular Weight: Theoretical: 41.99 daltons
Reaction & Storage Conditions

 Concentration: 500 mM
Storage Conditions: 1 X Sterile purified water
Storage Temperature: -20°C
References


- Hardie, D.G. et al. (1993) In D. G. Hardie (Ed.). Protein Phosphorylation: A Practical Approach, 62. New York: IRL Press.
- Gordon, J.A. (1991) Methods in Enzymology, 201, 477-482.
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