 |
|
 |
|
|
| Home >
Products >
Protein Tools >
Protein Phosphatases/Substrates/Inhibitors >
Sodium Orthovanadate (Vanadate) |
 | | | | Sodium Orthovanadate (Vanadate) | | | |
 |
|
Prices are in US dollars and valid only for US orders.
|
Description:
Sodium Orthovanadate (Vanadate, Na3VO4) is a commonly used general inhibitor for protein phosphotyrosyl phosphatases (PTPs). It is a competitive inhibitor. The inhibition by Vanadate is completely reversible upon the addition of EDTA or by dilution.
The PTP activities are conveniently separable from the protein phosphoseryl and phosphothreonyl phosphatase (PSP) activities inhibited by Fluoride (NEB# P0759) and EDTA. Routinely Vanadate is used to preserve the protein tyrosyl phosphorylation state in cells, cell lysates, and protein tyrosine kinase assays (1, 2).
Purity: >90% pure.
Suggested Working Concentration: 0.1-1 mM
Enzyme Properties
Molecular Weight:
Theoretical: 183.9 daltons
Reaction & Storage Conditions
Concentration:
100 mM
Storage Conditions:
1 X Sterile purified water
Storage Temperature:
-20°C
Notes
General notes:- Storage: Sterile purified water adjusted to pH 10.0 (1,2).
Usage notes:- Common buffer components such as EDTA and reducing agents may interact with Vanadate, affecting its potency (2).
100% of inhibition of NEB PTPs are found when the reaction buffer containing 25 mM Tris-HCl (pH 7.5 @ 25°C), 50 mM NaCl, 2 mM EDTA, 5 mM DTT, 0.01% Brij 35 is supplemented with 1 mM Vanadate.There is no inhibition of the same PTPs in the presence of 50 mM Fluoride under the same condition (3).
References
- Gordon, J.A. (1991) Methods in Enzymology, 201, 477-482.
- Huyer, G. et al. (1997) J. Biol. Chem., 272, 843-851.
- Barshevsky, T., unpublished observations.
Companion Products
LAR Protein Tyrosine Phosphatase (LAR)
T-Cell Protein Tyrosine Phosphatase (TC PTP)
YOP Protein Tyrosine Phosphatase (YOP PTP)
|
|
|
 |
|
|
