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Home > Products > Protein Tools > Protein Phosphatases/Substrates/Inhibitors > Adenosine 5´-Triphosphate (ATP) Adenosine 5´-Triphosphate (ATP) Catalog # Size Concentration Price Qty   P0756L 5 ml 10 mM $100.00 P0756S 1 ml 10 mM $25.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: Adenosine 5´-Triphosphate (ATP) serves as the phosphoryl donor in protein kinase assays, with apparent Km values for most protein kinases below 100 µM (1).  Purity: >99% pure.  Suggested Working Concentration:If possible, the ATP concentration should be at or near saturation (5 -10-fold over Km).  However, if the objective is to measure enzyme activity using gamma-labeled ATP, it is best to use 100-200 μM ATP in order to have higher specific activity of gamma-labeled ATP (100-500 cpm/pmol). Also, an excess of substrate should be used, and the level of phosphorylation should not exceed 10% for determination of the initial rate.  To phosphorylate a protein or peptide substrate to completion, the ATP concentration should be about 5-fold over the limited substrate concentration. Higher enzyme concentration and prolonged incubation times should be employed (1). Enzyme Properties Molecular Weight: Theoretical: 551.2 daltons Reaction & Storage Conditions Concentration: 10 mM Storage Conditions: 1 X Sterile purified water Storage Temperature: -20°C Notes General notes: Storage: Sterile purified water adjusted to pH 7.0 with NaOH. For long term storage, keep in small aliquos at -70°C. References Yang, Y. and Roach, P.J. (1993) In D.G. Hardie (Ed.). Protein Phosphorylation: A Practical Approach, 122-127. New York: IRL Press.