 |
|
 |
|
|
| Home >
Products >
Protein Tools >
Exoglycosidases >
β-N-Acetyl-hexosaminidasef |
 | | | | β-N-Acetyl-hexosaminidasef | | | |
 |
|
Prices are in US dollars and valid only for US orders.
|
Substrate Specificity:
Description:
β-N-Acetyl-hexosaminidasef is a recombinant protein fusion of β-N-Acetyl-hexosaminidase (1) and maltose binding protein. It has identical activity to β-N-Acetyl-hexosaminidase. β-N-Acetyl-hexosaminidasef catalyzes the hydrolysis of terminal β-D-N-acetyl-galactosamine and glucosamine residues from oligosaccharides.
Source:
Cloned from Streptomyces plicatus (1) and overexpressed in E. coli (2).
Reagents Supplied:
G2 Reaction Buffer (10X)
Enzyme Properties
Molecular Weight:
Theoretical: 100,000 daltons
Specific Activity:
10,000 units/mg
Reaction & Storage Conditions
Reaction Conditions:
1X G2 Reaction Buffer
Incubate at
37°C.
1X G2 Reaction Buffer:
50 mM sodium citrate
pH 4.5 @ 25°C
Unit Definition:
One unit is defined as the amount of enzyme required to cleave > 95% of the terminal β-D-N-acetyl-galactosamine from 1 nmol of GalNAcβ1-4Galβ1-4Glc-7-amino-4-methyl-coumarin (AMC), in 1 hour at 37°C in a total reaction volume of 10 µl.
Unit Definition Assay:
Two fold dilutions of β-N-Acetyl-hexosaminidasef are incubated with 1 nmol AMC-labeled substrate in 1X G2 Reaction Buffer in a 10 µl reaction. The reaction mix is incubated for 1 hour at 37°C. Separation of reaction products are visualized via thin layer chromatography (3).
Concentration:
5,000 units/ml
Storage Conditions:
20 mM Tris-HCl
50 mM NaCl
5 mM Na2EDTA
pH 7.5 @ 25°C
Storage Temperature:
-20°C
FAQs
- How much exoglycosidase should be used?
- Do detergents inhibit exoglycosidases/endoglycosidases?
- What is a good positive control for β-N-Acetylhexosaminidasef?
- Do detergents inhibit exoglycosidases/endoglycosidases?
- What are Glycosidases and their uses?
Quality Control for Current Lot
Quality control values for a specific lot can be found on the datacard which accompanies each product.
Quality Assurance Statement:
No contaminating exoglycosidase or proteolytic activity could be detected.
Protease Activity:
After incubation of 50 units of β-N-Acetyl-hexosaminidasef with a standard mixture of proteins
for 2 hours at 30ºC, no proteolytic activity could be detected by SDS-PAGE.
Exo/endoglycosidase Activity:
After incubation of 50 units of β-N-Acetyl-hexosaminidasef
with a variety of p-nitrophenyl-glycoside substrate and fluorescently-labeled oligosaccharides for 20 hours at 37ºC, no
contaminating exoglycosidase or endoglycosidase activities could be detected by spectrophotometric analysis or thin layer chromatography.
Contaminating activities tested for include ()
References
- Robbins, P. et al. (1992) Gene, 111, 69-76.
- Guan, C. and Wong, S., New England Biolabs, unpublished observations.
- Wong-Madden, S.T. and Landry D. (1995) Glycobiology, 5, 19-28.
Companion Products
Exoglycosidase Reaction Buffer Pack
|
|
|
 |
|
|
