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Protocols for pCLIP-H2B Control Plasmid FAQs for Gene Expression and Cellular Analysis Technical Reference for Gene Expression and Cellular Analysis Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder PCR Selection Tool Isoschizomers DNA Sequences and Maps REBASE

Home > Products > Gene Expression and Cellular Analysis > Cloning Vectors and Control Plasmids > pCLIP-H2B Control Plasmid pCLIP-H2B Control Plasmid Catalog # Size Concentration Price Qty   N9213S 20 μg   $125.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: This control plasmid contains the gene encoding the Histone H2B protein cloned upstream of the CLIP-tag™ coding sequence in pCLIP-tag™(m), as a fusion to the N- terminus of the CLIP-tag. Histone H2B is a member of the core histones that package DNA in the nucleus. The H2B-CLIP fusion protein gives nuclear fluorescence when labeled with CLIP-Cell substrates. The full sequence and map for pCLIP-H2B can be downloaded at www.neb.com.  The CLIP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The CLIP-tag is a small protein based on human O6-alkylguanine-DNA-alkyltransferase (AGT). CLIP-tag substrates are derivates of benzylcytosine (BC). In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the reactive cysteine of CLIP-tag forming a stable thioether link. Although CLIP-tag is based on the same protein as SNAP-tag®, the benzylcytosine substrates form a separate class of substrates, different from the benzylguanine substrates recognized by SNAP-tag. CLIP-tag and SNAP-tag can be used for orthogonal simultaneous labeling. There are two steps to using this system: sub-cloning and expression of the protein of interest as a CLIP-tag fusion, and labeling of the fusion with the CLIP-tag substrate of choice. Materials Required but not Supplied: Tissue culture media and reagents Mammalian cell lines Transfection reagents CLIP-tag substrates Live CHO-K1 cells transiently transfected with pCLIP-H2B. Cells were labeled with CLIP-Cell 505 (green) for 60 minutes and counterstained with Hoechst 33342 (blue). Reaction & Storage Conditions Storage Temperature: -20°C Notes Usage notes: Storage: pCLIP-H2B is supplied in TE buffer (10 mM Tris-HCl, pH 8.0, 1 mM EDTA) at a concentration of 0.5 µg/µl. Plasmid solutions can be stored at 4°C for up to one week. For long-term storage -20°C is recommended. Protocols Protocols for pCLIP-H2B Control Plasmid Legal Licenses/Patents/Disclaimers: Notice to Buyer/User: The Buyer/User has a non-exclusive license to use this system or any component thereof for RESEARCH AND DEVELOPMENT PURPOSES ONLY. Commercial use of this system or any components thereof requires a license from New England Biolabs, Inc., 240 County Road Ipswich, MA 01938. For detailed information, see www.neb.com/cia/legal/. New England Biolabs, Inc. is an ISO 9001 and ISO 14001 Certified Company. NEB certifies that it is a small business in accordance with the US Small Business Administration and 13 CFR 121.201