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RELATED INFORMATION Protocols for pSNAP-ADRB2 Control Plasmid FAQs for Gene Expression and Cellular Analysis Technical Reference for Gene Expression and Cellular Analysis FAVORITE TOOLS Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE

Home > Products > Gene Expression and Cellular Analysis > Cloning Vectors and Control Plasmids > pSNAP-ADRB2 Control Plasmid pSNAP-ADRB2 Control Plasmid Catalog # Size Concentration Price Qty   N9178S 20 μg   $125.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: This control plasmid contains the gene encoding the Beta-2 adrenergic receptor cloned as a fusion to the C-terminus of the SNAP-tag®. The signal peptide fused to the N-terminus of SNAP-tag is based on the 5HT3A serotonin receptor. The Beta-2 adrenergic receptor is a member of the G protein coupled receptors and mediates the catecholamine-induced activation of adenylate cyclase through the action of G proteins.  The SNAP-Beta-2 adrenergic receptor is inserted in the plasma membrane with the SNAP-tag exposed to the extracellular side of the membrane. When labeled with SNAP-tag substrates, it gives a selective cell membrane fluorescent labeling pattern.  The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a small protein based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). SNAP-tag substrates are derivates of benzyl purines and benzyl pyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.  There are two steps using of this system: sub-cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in this document. The labeling of the fusion proteins with SNAP-tag substrates is described in the instructions supplied with SNAP-tag substrates.  Materials Required but not Supplied: Cell culture media and reagents Mammalian cell lines Transfection reagents SNAP-tag substrates Live HEK293 cells transiently transfected with SNAP-ADRB2. Cells were labeled with SNAP-Surface™ 505 (green) 15 minutes. Reaction & Storage Conditions Storage Temperature: -20°C Notes Usage notes: Storage: pSNAP-ADRB2 is supplied in TE buffer (10 mM Tris-HCl, pH 8.0, 1 mM EDTA) at a concentration of 0.5 µg/µl. Plasmid solutions can be stored at 4°C for up to one week. For long-term storage -20°C is recommended. Protocols Protocols for pSNAP-ADRB2 Control Plasmid Legal Licenses/Patents/Disclaimers: Notice to Buyer/User: The Buyer/User has a non-exclusive license to use this system or any component thereof for RESEARCH AND DEVELOPMENT PURPOSES ONLY. Commercial use of this system or any components thereof requires a license from New England Biolabs, Inc., 240 County Road Ipswich, MA 01938. For detailed information, see www.neb.com/cia/legal/.