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Prices are in US dollars and valid only for US orders.
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Description:
The vector pMAL-c4X is designed to produce maltose-binding protein (MBP) fusions, where the protein of interest can be cleaved from MBP with the specific protease Factor Xa (NEB #P8010S).
MBP fusions made with this vector are expressed cytoplasmically. The MBP has been engineered for tighter binding to amylose resin.
A gene or open reading frame is inserted into a restriction site of the vector polylinker, in the same translational reading frame as the malE gene (encoding maltose-binding protein). Insertion of the DNA fragment interrupts the malE-lacZα fusion pre-existing on the vector, affording a screen for inserts on the proper indicator plates. The fusion protein thus produced can be purified by amylose affinity chromatography. The sequence coding for the four amino acids Ile-Glu-Gly-Arg is present just upstream of the XmnI site. This allows the protein of interest to be cleaved from maltose-binding protein with the specific protease Factor Xa. Fragments inserted in the XmnI site (cleaves GAAGG↓ATTTC) will produce a fusion protein that, after Factor Xa cleavage, contains no vector-derived residues on the protein of interest.
Source:
NEB 5-alpha Competent E. coli (pMAL-c4X)
Concentration:
200 μg/ml
Storage Conditions
Storage Conditions:
10 mM Tris-HCl
1 mM EDTA
pH 7.5 @ 25°C
Storage Temperature:
-20°C
FAQs
- Why did NEB switch from selling the pMAL-p2 and c2 series of vectors to the pMAL p4 and c4 series?
References
- Guan, C., Li, P., Riggs, P. D. and Inouye, H. (1987) Gene, 67, 21-30.
- Maina, C. V., Riggs, P. D., Grandea, A. G. III, Slatko, B. E., Moran, L. S., Tagliamonte, J. A., McReynolds, L. A. and Guan, C. (1988) Gene, 74, 365-373.
- Nagai, K. and Thogersen, H. C. (1987) Methods Enzymology, 153, 461-481.
- Riggs, P. D. (1990) Expression and Purification of Maltose-Binding Protein Fusions. In F. M. Ausebel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith and K. Struhl (Eds.). Current Protocols in Molecular Biology, 16.6.1-16.6.12.
- Yanisch-Perron, C., Vieira, J. and Messing, J. (1985) Gene, 33, 103-119.
Legal
Patents:
New England Biolabs, Inc.: U.S. Patent No. 5,643,758
Research Use Assurance:
The buyer/user has a non-exclusive license to use the pMAL vectors for research purposes only. A license to use the pMAL vectors for commercial purposes is available from New England Biolabs, Inc.
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