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Protocols for pMAL-pIII Vector FAQs for Protein Expression and Purification Technical Reference for Protein Expression and Purification Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder PCR Selection Tool Isoschizomers DNA Sequences and Maps REBASE Amylose Resin Anti-MBP Monoclonal Antibody Ph.D.™ Peptide Display Cloning System Ph.D.™-12 Phage Display Peptide Library Ph.D.™-7 Phage Display Peptide Library Ph.D.™-C7C Phage Display Peptide Library

Home > Products > Protein Expression and Purification > pMAL Companion Products > pMAL-pIII Vector pMAL-pIII Vector Catalog # Size Concentration Price Qty   N8101S 10 μg 500 μg/ml $79.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: The pMal-pIII Vector is a derivative of pMal-p2 in which the leader sequence of maltose binding protein (MBP, malE) has been replaced with the M13 pIII leader sequence. KpnI/Acc65I and EagI sites have been introduced within the pIII leader to facilitate direct transfer of sequences selected from any of the Ph.D. phage display peptide libraries into an expression vector. The corresponding peptides are expressed as N-terminal MBP fusions. These fusions can then be easily purified from E. coli periplasmic space by osmotic shock followed by affinity chromatography on amylose resin. Since the peptide is expressed at the N-terminus of MBP, Factor Xa cannot be used to cleave the peptide from MBP. pMal-pIII Plasmid Map. Restriction sites used for subcloning fragments from PhD libraries into pMal-pIII are shown in red. pMal-pIII cloning region. Note PhD fragments are inserted into the M13KE pIII leader sequence, which is directly followed by the malE gene. Source: Isolated from E. coli strain ER2272 by standard DNA purification procedure. Advantages: Rapid characterization of selected sequences by ELISA Alternative to chemical peptide synthesis Monovalency of MBP fusion allows accurate KD determination Concentration: 500 μg/ml Storage Conditions Storage Conditions: 10 mM Tris-HCl 1 mM EDTA pH 7.5 @ 25°C Storage Temperature: -20°C Protocols Protocols for pMAL-pIII Vector References Zwick, M. B. et al (1998) Anal. Biochem., 264, 87-97. Zagursky, R.J. et al (1984) Gene, 27, 193-91. Companion Products Amylose Resin Anti-MBP Monoclonal Antibody Ph.D.™ Peptide Display Cloning System Ph.D.™-12 Phage Display Peptide Library Ph.D.™-7 Phage Display Peptide Library Ph.D.™-C7C Phage Display Peptide Library New England Biolabs, Inc. is an ISO 9001 and ISO 14001 Certified Company. NEB certifies that it is a small business in accordance with the US Small Business Administration and 13 CFR 121.201