 | | | | pNEBR-R1 Regulator Plasmid | | | |
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Prices are in US dollars and valid only for US orders.
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- Ampicillin resistance for propagation in E. coli.
- Neomycin resistance for selection in mamalian cells.
- Can be linearized using one of the unique restriction enzyme sites shown on map.
Description:
pNEBR-R1 is designed to control gene expression in mammalian cells as part of the RheoSwitch® Inducible Gene Expression System (NEB #E3000). The plasmid expresses an engineered nuclear receptor heterodimer consisting of two proteins, RheoReceptor-1 and RheoActivator. The two proteins constitute the holoreceptor and regulate transcription of genes cloned into the expression vector, pNEBR-X1 (NEB #N8078). The two proteins are expressed from the constitutive ubiquitin UbC and UbB promoters, respectively. The plasmid also contains the neomycin resistance gene under control of the SV40 early promoter for the generation of stable cell lines.
Source:
pNEBR-R1 is isolated from E. coli strain ER2272 by a standard plasmid purification procedure.
Concentration:
500 μg/ml
Storage Conditions
Storage Conditions:
10 mM Tris-HCl
1 mM EDTA
pH 7.5 @ 25°C
Storage Temperature:
-20°C
pNEBR-R1 Regulator Plasmid map. RheoReceptor-1 and RheoActivator proteins are expressed from the constitutive UbC and UbB promoters, respectively. The plasmid also contains the neomycin resistance gene under control of the SV40 early promoter for the generation of stable cell lines. Unique restriction enzyme sites are shown.
References
- RheoSwitch Mammalian Inducible Expression System Manual, unpublished observations.
- Palli, S.R., Kapitskaya, M.Z., Kumar, M.B. and Cress, D.E. (2003) Eur. J. Biochem., 270, 1308-1315.
- Dhadialla, T.S., Carlson, G.R. and Le, D.P. (1998) Annu. Rev. Entomol., 43, 545-569.
- Kumar, M.B., Potter, D.W., Hormann, R.E., Edwards, A., Tice, C.M., Smith, H.C., Dipietro, M.A., Polley, M., Lawless, M., Wolohan, P.R., Kethidi, D.R. and Palli, S.R. (2004) J. Biol. Chem., 279, 27211-27218.
- Verhaegent, M. and Christopoulos, T.K. (2002) Anal. Chem., 74, 4378-4385.
Companion Products
pNEBR-X1 Vector
pNEBR-X1GLuc Control Plasmid
pNEBR-X1Hygro Vector
RheoSwitch® Ligand RSL1
RheoSwitch® Mammalian Inducible Expression System
TransPass™ D1 Transfection Reagent
TransPass™ D2 Transfection Reagent
Legal
Patents:
RheoGene: U.S. Patent No. 6,258, 603
RheoGene: U.S. Patent No. 5,514,578
RheoGene: U.S. Patent No. 6,245,531
RheoGene: U.S. Patent No. 5,530,082
Licenses/Patents/Disclaimers:
This product is the subject of one or more of U.S. Patent Nos. 5,514,578, 6,245,531, including the corresponding foreign patents (Australian Patent No. 752699, Canadian Patent App No. 2076386, European Patent No. 0517805) and patent applications, U.S. Patent Nos. 6,258,603, 5,530,028 and U.S. Patent Applications 09/965,703, 10/239,134, 10/468,199, 10/468,193, licensed to New England Biolabs.
The purchase of this product conveys to the non-commercial purchaser the non-transferable right to use the purchased amount of the product and components of the product for research use only conducted by the purchaser. For commercial purchasers using this product for research, the purchase of this product conveys to the purchaser the non-transferable right to use the purchased amount of the product and components of the product for a six(6) month evaluation period after the date of purchase for research use only. After the six(6) month evaluation period, the purchaser must contact RheoGene, Inc. to obtain a commercial research license. The purchaser cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for commercial purposes. The purchaser cannot use this product or its components in whole plants, plant cells or humans. The purchaser cannot directly or indirectly disassemble, decompile, translate, reverse engineer, modify or adapt, (a) this product or (b) its components. The purchaser may transfer information or materials made through the use of this product to a scientific collaborator, provided that such transfer is not for the commercial purposes of the purchaser, and that such collaborator agrees in writing (a) not to transfer such materials to any third party, and (b) to use such transferred materials and/or information solely for research and not for commercial purposes.
Commercial purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data (3) use of the products or its components for therapeutic, diagnostic or prophylactic purposes; (4) use of the product or its components to screen for agonists to the RheoSwitch® System; or (5) resale of the product or its components, whether or not such product or its components are resold for use in research. If the purchaser is not willing to accept the limitations of the limited use statement, NEB is willing to accept return of the products with a full refund. For information on commercial use of this product or obtaining a license to this product for purposes other than research, contact RheoGene, Inc. by calling Dr. Camille Jolly-Tornetta, Director of Intellectual Property (610-650-8734, ext. 104).
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