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Biotinylated 2-Log DNA Ladder (0.1-10 kb) |
 | | | | Biotinylated 2-Log DNA Ladder (0.1-10 kb) | | | |
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Prices are in US dollars and valid only for US orders.
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Description:
A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 19 bands and subsequently biotinylated for use as molecular weight standards for chemiluminescent detection. All bands have equal intensity except for the 0.5, 1.0 and 3.0 kb bands which have increased intensity to serve as reference points.
1.0 µg of 2-Log DNA Ladder visualized by ethidium bromide staining on a 1.0% TBE agarose gel.
Effective Size Range: 100 bp to 10 kb
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| 1 | | 10,002 | | 40 | | |
| 2 | | 8,001 | | 40 | | |
| 3 | | 6,001 | | 48 | | |
| 4 | | 5,001 | | 40 | | |
| 5 | | 4,001 | | 32 | | |
| 6 | | 3,001 | | 120 | | |
| 7 | | 2,017 | | 40 | | |
| 8 | | 1,517 | | 57 | | |
| 9 | | 1,200 | | 45 | | |
| 10 | | 1,000 | | 122 | | |
| 11 | | 900 | | 34 | | |
| 12 | | 800 | | 31 | | |
| 13 | | 700 | | 27 | | |
| 14 | | 600 | | 23 | | |
| 15a | | 517 | | 124 | | |
| 15b | | 500 | | 124 | | |
| 16 | | 400 | | 49 | | |
| 17 | | 300 | | 37 | | |
| 18 | | 200 | | 32 | | |
| 19 | | 100 | | 61 | | |
Concentration:
1,000 μg/ml
Recommended Load: 1 μg
Storage Conditions
Storage Conditions:
10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C
Storage Temperature:
-20°C
Notes
General notes:- 1 µl of marker per gel lane is usually sufficient to view the DNA fragments using standard chemiluminescent detection.
FAQs
- What are the overhangs on the DNA ladder fragments? Can I end-label them using the T4 polynucleotide kinase (PNK)? What about the Klenow fragment?
- Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
- How can I quantify the amount of DNA in each band of a marker?
Companion Products
1 kb DNA Ladder
100 bp DNA Ladder
2-Log DNA Ladder (0.1–10.0 kb)
PCR Marker
TriDye™ 1 kb DNA Ladder
TriDye™ 100 bp DNA Ladder
TriDye™ 2-Log DNA Ladder (0.1 - 10.0 kb)
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