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FAQs for Discontinued Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder PCR Selection Tool Isoschizomers DNA Sequences and Maps REBASE GPS™ Buffer Pack GPS™-1 Genome Priming System GPS™-LS Linker Scanning System GPS™-M Mutagenesis System pGPS1.1 Transprimer Donor pGPS2.1 Transprimer Donor pGPS4 Transprimer Donor pGPS5 Transprimer Donor TnsABC* Transposase

Home > Products > Discontinued > Discontinued > pGPS3 Transprimer Donor pGPS3 Transprimer Donor Discontinued as of 4/1/09. Product is no longer available. This product is not available for online ordering. Download: MSDS PDF Description: pGPS3 is the Transprimer-1 donor plasmid used in the GPS™-M Mutagenesis System (NEB #E7101S). TnsABC* Transposase removes the Transprimer-1 element from pGPS3 and inserts it randomly into the target DNA in vitro (1-3). Transprimer-1 encodes kanamycin-resistance. This double-stranded plasmid is 4293 bp in size. Source: pGPS3 is isolated from E. coli ER2502 Concentration: 200 μg/ml Storage Conditions Storage Conditions: 10 mM Tris-HCl 1 mM EDTA pH 8.0 @ 25°C Storage Temperature: -20°C Quality Control for Current Lot Quality control values for a specific lot can be found on the datacard which accompanies each product. GPS Control Reaction:: A 20 µl GPS Control Reaction was performed using 0.02 µg pGPS3, 0.08 µg Control Target plasmid and 1 µl TnsABC* Transposase in 1X GPS Buffer supplemented with 15 mM Start Solution after 10 minutes. This was incubated for 1 hour at 37°C and transformed into chemically-competent E. coli (1 x 107 per µg pBR322). Less than 1% of the resulting kanamycin-resistant colonies contained cointegrate molecules. References Craig, N.L. (1996) Curr. Top. Microbiol. Immunol., 204, 27-48. Stellwagen, A.E. and Craig, N.L. (1997) Genetics, 145, 573-585. Biery, M.C., Stewart, F.J., Stellwagen, A.E., Raleigh, E.A. and Craig, N.L. (2000) Nucl. Acids Res., 28, 1067-1077. Companion Products GPS™-1 Genome Priming System GPS™-LS Linker Scanning System GPS™-M Mutagenesis System pGPS1.1 Transprimer Donor pGPS2.1 Transprimer Donor pGPS4 Transprimer Donor pGPS5 Transprimer Donor TnsABC* Transposase Legal Patents: John Hopkins University: Licensed Under U.S. Patent No. 6,420,524 New England Biolabs, Inc. is an ISO 9001 and ISO 14001 Certified Company. NEB certifies that it is a small business in accordance with the US Small Business Administration and 13 CFR 121.201