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Prices are in US dollars and valid only for US orders.
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Description:
pGPS3 is the Transprimer-1 donor plasmid used in the GPS™-M Mutagenesis System (NEB #E7101S). TnsABC* Transposase removes the Transprimer-1 element from pGPS3 and inserts it randomly into the target DNA in vitro (1-3). Transprimer-1 encodes kanamycin-resistance. This double-stranded plasmid is 4293 bp in size.
Source:
pGPS3 is isolated from E. coli ER2502
Concentration:
200 μg/ml
Storage Conditions
Storage Conditions:
10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C
Storage Temperature:
-20°C
Quality Control for Current Lot
Quality control values for a specific lot can be found on the datacard which accompanies each product.
GPS Control Reaction::
A 20 µl GPS Control Reaction was performed using 0.02 µg pGPS3, 0.08 µg Control Target plasmid and 1 µl TnsABC* Transposase in 1X GPS Buffer supplemented with 15 mM Start Solution after 10 minutes. This was incubated for 1 hour at 37°C and transformed into chemically-competent E. coli (1 x 107 per µg pBR322). Less than 1% of the resulting kanamycin-resistant colonies contained cointegrate molecules.
References
- Craig, N.L. (1996) Curr. Top. Microbiol. Immunol., 204, 27-48.
- Stellwagen, A.E. and Craig, N.L. (1997) Genetics, 145, 573-585.
- Biery, M.C., Stewart, F.J., Stellwagen, A.E., Raleigh, E.A. and Craig, N.L. (2000) Nucl. Acids Res., 28, 1067-1077.
Companion Products
GPS™ Buffer Pack
GPS™-1 Genome Priming System
GPS™-LS Linker Scanning System
GPS™-M Mutagenesis System
pGPS1.1 Transprimer Donor
pGPS2.1 Transprimer Donor
pGPS4 Transprimer Donor
pGPS5 Transprimer Donor
TnsABC* Transposase
Legal
Patents:
John Hopkins University: Licensed Under U.S. Patent No. 6,420,524
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