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CDP-Star® Reagent |
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Prices are in US dollars and valid only for US orders.
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Description:
CDP-Star Reagent is a 1,2-dioxetane compound utilized in Phototope® alkaline phosphatase-based chemiluminescent detection assays. CDP-Star Reagent produces a light signal when it is activated by alkaline phosphatase, accumulates in its dephosphorylated form and decomposes at a constant rate for up to several days. When assayed on nylon membrane at 1:100 in the buffer provided, CDP-Star signals reach a maximum within 15 minutes and decay slowly over 3 days. Typical film exposure times range from 15 seconds to 15 minutes.
Reaction Conditions:
Detection of alkaline phosphatase on membranes as follows:
1. Wash membrane-bound alkaline phosphatase (biotin or antibody conjugate) in 10 mM Tris-HCl, 10 mM NaCl, 1 mM MgCl2 (pH 9.5).
2. Dilute CDP-Star Reagent 1:100 to 1:500 in 1X CDP-Star Assay Buffer (0.025-0.1 ml/cm2) and incubate with membrane for 5 minutes at room temperature.
3. Remove excess solution (do not allow membrane to dry), wrap in plastic or reseal hybridization bag and expose to X-ray film.
Preparation of Solutions:
Use only Milli-Q™ or equivalent water.
Reagents Supplied:
CDP-Star® Assay Buffer (25X)
Reaction & Storage Conditions
Concentration:
25 mM
Storage Temperature:
4°C
Notes
Usage notes:- Use only Milli-Q or equivalent water.
Reagents Sold Separately
CDP-Star® Assay Buffer
Companion Products
Phototope®-Star Detection Kit
Legal
Patents:
New England Biolabs, Inc.: U.S. 5,326,882
New England Biolabs, Inc.: U.S. 4,931,569
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